Pyrogallol inhibits the growth of lung cancer Calu-6 cells via caspase-dependent apoptosis

被引：49
作者：
Han, Yong Hwan [1 ]
Kim, Sung Zoo [1 ]
Kim, Suhn Hee [1 ]
Park, Woo Hyun [1 ]
机构：
[1] Chonnam Natl Univ, Inst Med Sci, Ctr Healthcare Technol Dev, Dept Physiol,Med Sch, Jeonju 561180, South Korea
来源：
CHEMICO-BIOLOGICAL INTERACTIONS | 2009年 / 177卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
Pyrogallol; Apoptosis; Calu-6; Caspase; ROS; AS4.1 JUXTAGLOMERULAR CELLS; CYCLE ARREST; SUPEROXIDE ANION; ROS; DEATH; ACTIVATION; MECHANISMS; GENERATOR; ROLES; P53;
D O I：
10.1016/j.cbi.2008.10.014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pyrogallol (PG) is a polyphenol compound and a known O-2(center dot-) generator. We evaluated the effects of PC on the growth and apoptosis of human pulmonary adenocarcinoma Calu-6 cells. PC decreased the viability of Calu-6 cells in a dose- and time-dependent manner. The induction of apoptosis by PC was accompanied by the loss of mitochondrial membrane potential (Delta psi(m)), cytochrome c release from mitochondria and activation of caspase-3 and caspase-8. All tested caspase inhibitors, especially the pan-caspase inhibitor (Z-VAD), markedly rescued Calu-6 cells from PC-induced cell death. Rescue was accompanied by inhibition of caspase-3 activation and PARP cleavage. Treatment with Z-VAD also prevented the loss of mitochondrial membrane potential (Delta psi(m)) In conclusion, PC inhibits the growth of Calu-6 cells via caspase-dependent apoptosis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：107 / 114
页数：8
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