Pyrogallol inhibits the growth of lung cancer Calu-6 cells via caspase-dependent apoptosis

被引：49

作者：

Han, Yong Hwan ^{[1
]}

Kim, Sung Zoo ^{[1
]}

Kim, Suhn Hee ^{[1
]}

Park, Woo Hyun ^{[1
]}

机构：

[1] Chonnam Natl Univ, Inst Med Sci, Ctr Healthcare Technol Dev, Dept Physiol,Med Sch, Jeonju 561180, South Korea

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2009年 / 177卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Pyrogallol; Apoptosis; Calu-6; Caspase; ROS; AS4.1 JUXTAGLOMERULAR CELLS; CYCLE ARREST; SUPEROXIDE ANION; ROS; DEATH; ACTIVATION; MECHANISMS; GENERATOR; ROLES; P53;

D O I：

10.1016/j.cbi.2008.10.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pyrogallol (PG) is a polyphenol compound and a known O-2(center dot-) generator. We evaluated the effects of PC on the growth and apoptosis of human pulmonary adenocarcinoma Calu-6 cells. PC decreased the viability of Calu-6 cells in a dose- and time-dependent manner. The induction of apoptosis by PC was accompanied by the loss of mitochondrial membrane potential (Delta psi(m)), cytochrome c release from mitochondria and activation of caspase-3 and caspase-8. All tested caspase inhibitors, especially the pan-caspase inhibitor (Z-VAD), markedly rescued Calu-6 cells from PC-induced cell death. Rescue was accompanied by inhibition of caspase-3 activation and PARP cleavage. Treatment with Z-VAD also prevented the loss of mitochondrial membrane potential (Delta psi(m)) In conclusion, PC inhibits the growth of Calu-6 cells via caspase-dependent apoptosis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：107 / 114

页数：8

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