Development and evaluation of a Salmonella typhimurium flagellin based chimeric DNA vaccine against infectious bursal disease of poultry

被引：9

作者：

Deb, Rajib ^{[1
]}

Dey, Sohini ^{[1
]}

Mohan, C. Madhan ^{[1
]}

Gaikwad, Satish ^{[1
]}

Kamble, Nitin ^{[1
]}

Khulape, Sagar A. ^{[1
]}

Gupta, Shishir Kumar ^{[1
]}

Maity, Hemanta Kumar ^{[1
]}

Pathak, Dinesh Chandra ^{[1
]}

机构：

[1] Indian Vet Res Inst, Recombinant DNA Lab, Div Vet Biotechnol, Bareilly 243122, Uttar Pradesh, India

来源：

RESEARCH IN VETERINARY SCIENCE | 2015年 / 102卷

关键词：

IBDV; VP2; fliC; Salmonella; Poultry; DNA vaccine; INACTIVATED INFLUENZA VACCINE; NF-KAPPA-B; IMMUNE-RESPONSES; MEDIATED VACCINATION; VP2; GENE; MYCOBACTERIUM-TUBERCULOSIS; BACTERIAL FLAGELLIN; ADJUVANT ACTIVITY; RECEPTOR LIGANDS; INTERFERON-GAMMA;

D O I：

10.1016/j.rvsc.2015.07.004

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Infectious bursal disease (IBD) is an acute immunosuppressive disease of young chicks, caused by a double-stranded RNA virus. VP2 being the major capsid protein of the virus is an ideal vaccine candidate possessing the neutralizing epitopes. The present study involves the use of flagellin (fliC) as a genetic adjuvant to improve the immune response of VP2 based DNA vaccine against IBD. Our findings revealed that birds immunized with plasmid pCIVP2fliC showed robust immune response than pCIVP2 immunized groups. Further, challenge study proved that genetic fusion of fliC and VP2 can provide a comparatively higher level of protection against vvIBDV challenge in chickens than VP2 alone. These results thus indicate that Salmonella flagellin could enhance the immune responses and protection efficacy of a DNA vaccine candidate against IBDV infection in chickens, highlighting the potential of flagellin as a genetic adjuvant in the prevention of vvIBDV infection. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：7 / 14

页数：8

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