Efficient experimental design and analysis of real-time PCR assays

被引：4
作者：
Hui, Kwokyin [1 ]
Feng, Zhong-Ping [1 ]
机构：
[1] Univ Toronto, Fac Med, Dept Physiol, Toronto, ON M5S 1A8, Canada
来源：
CHANNELS | 2013年 / 7卷 / 03期
基金：
加拿大自然科学与工程研究理事会;
关键词：
hypertrophy; ANF; L-type calcium channel; T-type calcium channel; gene expression; Real-time PCR; ventricular myocytes; cell culture; POLYMERASE-CHAIN-REACTION; VENTRICULAR CELLS; EXPRESSION; STIMULATION; ACTIVATION; CHANNELS; RECEPTOR; ALPHA; GENE;
D O I：
10.4161/chan.24024
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Real-time polymerase chain reaction (qPCR) is currently the standard for gene quantification studies and has been extensively used in large-scale basic and clinical research. The operational costs and technical errors can become a significant issue due to the large number of sample reactions. In this paper, we present an experimental design strategy and an analysis procedure that are more efficient requiring fewer sample reactions than the traditional approach. We verified mathematically and experimentally the new design on a well-characterized model, to evaluate the gene expression levels of CACNA1C and CACNA1G in hypertrophic ventricular myocytes induced by phenylephrine treatment.
引用
收藏
页码：160 / 170
页数：11
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