Genetic transformation of Coffea canephora by vacuum infiltration

被引：25

作者：

Canche-Moo, RLR

Ku-Gonzalez, A

Burgeff, C

Loyola-Vargas, VM

Rodríguez-Zapata, LC

Castaño, E

机构：

[1] Ctr Invest Cient Yucatan, Unidad Bioquim & Biol Mol Plantas, Merida 97200, Yucatan, Mexico

[2] Ctr Invest Cient Yucatan, Unidad Biotecnol, Merida 97200, Yucatan, Mexico

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2006年 / 84卷 / 03期

关键词：

Agrobacterium transformation; coffee; somatic embryogenesis; vacuum infiltration;

D O I：

10.1007/s11240-005-9036-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Agrobacterium-mediated plant transformation protocol was evaluated as a fast method to obtain genetically modified Coffea canephora plantlets. Leaf explants were used as source material for Agrobacterium tumefaciens-mediated transformation involving a vacuum infiltration protocol, followed by a step of somatic embryogenesis induction and a final selection of the transformed plants. A. tumefaciens strain C58CI containing the binary vector pER10W-35SRed was used. PCR amplification of DsRFP gene and visual detection of the red fluorescent protein demonstrated 33% transformed embryos. The protocol presented here produces reliable transgenic coffee embryos in two months.

引用

页码：373 / 377

页数：5

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