Fluorescence Lifetime Imaging of Biosensor Peptide Phosphorylation in Single Live Cells

被引：37
作者：
Damayanti, Nur P. [1 ]
Parker, Laurie L. [2 ]
Irudayaraj, Joseph M. K. [1 ]
机构：
[1] Purdue Univ, Bindley Biosci Ctr, Purdue Ctr Canc Res, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Coll Pharm, Purdue Ctr Canc Res, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 14期
基金：
美国国家卫生研究院;
关键词：
Abl kinase; biosensors; fluorescence lifetime imaging microscopy; fluorescent probes; inhibitors; KINASE-ACTIVITY; CORRELATION SPECTROSCOPY; PROTEIN-KINASES; VISUALIZATION; REPORTERS; KINOME;
D O I：
10.1002/anie.201209303
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Better living through biochemistry: Phosphorylation of a Cy5-labeled Abl kinase peptide biosensor resulted in an extension of fluorescence lifetime in live cells. Time-correlated single photon counting fluorescence lifetime imaging (FLIM) enabled excellent signal-to-noise to visualize the subcellular patterns of this sensor (see picture). This strategy should be generalizable to other peptide-based kinase substrates for imaging of kinase activity in single cells. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：3931 / 3934
页数：4
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