The H19 Imprinting Control Region Mediates Preimplantation Imprinted Methylation of Nearby Sequences in Yeast Artificial Chromosome Transgenic Mice
被引:11
作者:
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Okamura, Eiichi
[1
]
Matsuzaki, Hitomi
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Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki, JapanUniv Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan
Matsuzaki, Hitomi
[2
]
Sakaguchi, Ryuuta
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Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, JapanUniv Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan
Sakaguchi, Ryuuta
[1
]
Takahashi, Takuya
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Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, JapanUniv Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan
Takahashi, Takuya
[1
]
Fukamizu, Akiyoshi
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Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki, Japan
Univ Tsukuba, Life Sci Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki, JapanUniv Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan
Fukamizu, Akiyoshi
[2
,3
]
Tanimoto, Keiji
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Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki, Japan
Univ Tsukuba, Life Sci Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki, JapanUniv Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan
Tanimoto, Keiji
[2
,3
]
机构:
[1] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan
[2] Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki, Japan
[3] Univ Tsukuba, Life Sci Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki, Japan
ENHANCER-BLOCKING ACTIVITY;
DE-NOVO METHYLATION;
BETA-GLOBIN LOCUS;
HISTONE H3 METHYLTRANSFERASE;
BECKWITH-WIEDEMANN SYNDROME;
MALE GERM-CELLS;
DNA METHYLATION;
FUNCTIONAL-CHARACTERIZATION;
BINDING SEQUENCES;
ERYTHROID-CELLS;
D O I:
10.1128/MCB.01003-12
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
In the mouse Igf2/H19 imprinted locus, differential methylation of the imprinting control region (H19 ICR) is established during spermatogenesis and is maintained in offspring throughout development. Previously, however, we observed that the paternal H19 ICR, when analyzed in yeast artificial chromosome transgenic mice (YAC-TgM), was preferentially methylated only after fertilization. To identify the DNA sequences that confer methylation imprinting, we divided the H19 ICR into two fragments (1.7 and 1.2 kb), ligated them to both ends of a lambda DNA fragment into which CTCF binding sites had been inserted, and analyzed this in YAC-TgM. The maternally inherited lambda sequence, normally methylated after implantation in the absence of H19 ICR sequences, became hypomethylated, demonstrating protective activity against methylation within the ICR. Meanwhile, the paternally inherited lambda sequence was hypermethylated before implantation only when a 1.7-kb fragment was ligated. Consistently, when two subfragments of the H19 ICR were individually investigated for their activities in YAC-TgM, only the 1.7-kb fragment was capable of introducing paternal allele-specific DNA methylation. These results show that postfertilization methylation imprinting is conferred by a paternal allele-specific methylation activity present in a 1.7-kb DNA fragment of the H19 ICR, while maternal allele-specific activities protect the allele from de novo DNA methylation.
机构:
Univ Leeds, Leeds Inst Genet & Hlth Therapeut, Reprod & Early Dev Unit, Leeds LS2 9JT, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Arpanahi, Ali
;
Brinkworth, Martin
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Univ Bradford, Sch Life Sci, Bradford BD7 1DP, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Brinkworth, Martin
;
Iles, David
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Univ Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Iles, David
;
Krawetz, Stephen A.
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机构:
Wayne State Univ, Sch Med, Dept Obstet & Gynecol, Ctr Mol Med & Genet, Detroit, MI 48201 USAUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Krawetz, Stephen A.
;
Paradowska, Agnieszka
论文数: 0引用数: 0
h-index: 0
机构:
Univ Giessen, Dept Urol & Pediat Urol, D-35385 Giessen, GermanyUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Paradowska, Agnieszka
;
Platts, Adrian E.
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机构:
Wayne State Univ, Sch Med, Dept Obstet & Gynecol, Ctr Mol Med & Genet, Detroit, MI 48201 USAUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Platts, Adrian E.
;
Saida, Myriam
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机构:
Univ Leeds, Leeds Inst Genet & Hlth Therapeut, Reprod & Early Dev Unit, Leeds LS2 9JT, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Saida, Myriam
;
Steger, Klaus
论文数: 0引用数: 0
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机构:
Univ Giessen, Dept Urol & Pediat Urol, D-35385 Giessen, GermanyUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Steger, Klaus
;
Tedder, Philip
论文数: 0引用数: 0
h-index: 0
机构:
Univ Leeds, Inst Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
机构:
Univ Leeds, Leeds Inst Genet & Hlth Therapeut, Reprod & Early Dev Unit, Leeds LS2 9JT, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Arpanahi, Ali
;
Brinkworth, Martin
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bradford, Sch Life Sci, Bradford BD7 1DP, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Brinkworth, Martin
;
Iles, David
论文数: 0引用数: 0
h-index: 0
机构:
Univ Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Iles, David
;
Krawetz, Stephen A.
论文数: 0引用数: 0
h-index: 0
机构:
Wayne State Univ, Sch Med, Dept Obstet & Gynecol, Ctr Mol Med & Genet, Detroit, MI 48201 USAUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Krawetz, Stephen A.
;
Paradowska, Agnieszka
论文数: 0引用数: 0
h-index: 0
机构:
Univ Giessen, Dept Urol & Pediat Urol, D-35385 Giessen, GermanyUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Paradowska, Agnieszka
;
Platts, Adrian E.
论文数: 0引用数: 0
h-index: 0
机构:
Wayne State Univ, Sch Med, Dept Obstet & Gynecol, Ctr Mol Med & Genet, Detroit, MI 48201 USAUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Platts, Adrian E.
;
Saida, Myriam
论文数: 0引用数: 0
h-index: 0
机构:
Univ Leeds, Leeds Inst Genet & Hlth Therapeut, Reprod & Early Dev Unit, Leeds LS2 9JT, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Saida, Myriam
;
Steger, Klaus
论文数: 0引用数: 0
h-index: 0
机构:
Univ Giessen, Dept Urol & Pediat Urol, D-35385 Giessen, GermanyUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England
Steger, Klaus
;
Tedder, Philip
论文数: 0引用数: 0
h-index: 0
机构:
Univ Leeds, Inst Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, EnglandUniv Leeds, Fac Biol Sci, Inst Integrat & Comparat Biol, Leeds LS2 9JT, W Yorkshire, England