Sanzi Yangqin Decoction Alleviates Allergic Asthma by Modulating Th1/Th2 Balance: Coupling Network Pharmacology with Biochemical Pharmacology

被引：7

作者：

Peng, Wenpan ^{[1
]}

Xu, Yong ^{[2
]}

Zhou, Yunhai ^{[1
]}

Wu, Juanjuan ^{[1
]}

Peng, Guiqing ^{[1
]}

Gu, Yanlan ^{[1
]}

Zhou, Xianmei ^{[3
]}

Pu, Mingzhi ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Suzhou TCM Hosp, Suzhou 215000, Peoples R China

[2] Nanjing Univ Chinese Med, Sch Chinese Med, Sch Integrated Chinese & Western Med, Nanjing, Peoples R China

[3] Jiangsu Prov Hosp Chinese Med, Dept Resp Med, Nanjing 210029, Peoples R China

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2022年 / 2022卷

基金：

中国国家自然科学基金;

关键词：

INNATE IMMUNE-RESPONSES; BRONCHOALVEOLAR LAVAGE; TH2; CYTOKINES; CUTTING EDGE; IFN-BETA; CELLS; PATHOGENESIS; RHINOVIRUS; RECEPTOR; INTERLEUKIN-4;

D O I：

10.1155/2022/9037154

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

This study aimed to verify that Sanzi Yangqin Decoction (SYD) can relieve asthma in mice and explore the effect on TH1/Th2 balance. The targets of SYD and asthma were explored from the public database using various methods. The potential targets and signaling pathways were identified by KEGG enrichment analysis from DAVID database. Mice asthma models were established using OVA and aluminum hydroxide. Lung tissues of mice were stained with HE and Masson. The contents of IFN-gamma, IL-4, and TNF-alpha in BALF and IgE in mouse serum were detected using ELISA. In addition, the changes in Th1 and Th2 cells of the spleen were detected by flow cytometry. Fourteen core targets including IL4, IFNG, and MMP9 were identified for the treatment of asthma by SYD. The content of IL-4 in the lung tissue and BALF was gradually decreased with the increase in SYD concentration, while the IFN-gamma was gradually increased. The drug significantly reduced IgE levels in serum and TNF-alpha in BALF. The number of Th1 cells in the spleen increased, while Th2 cells decreased in a concentration-dependent manner. SYD can alleviate pulmonary inflammation, restore Th1/Th2 balance, and relieve asthma.

引用

页数：13

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