Determination of Carbohydrates by High Performance Anion Chromatography-Pulsed Amperometric Detection in Mushrooms

被引：11
作者：
Zhou, Shuai
Tang, Qing-Jiu [1 ]
Luo, Xi
Xue, Jun-Jie
Liu, Yan-Fang
Yang, Yan
Zhang, Jin-Song
Feng, Na
机构：
[1] Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS | 2012年 / 14卷 / 04期
关键词：
medicinal mushrooms; high performance anion chromatography; pulsed amperometric detection; trehalose; mannitol; arabitol; glucose; carbohydrates; NONVOLATILE TASTE COMPONENTS; WILD EDIBLE MUSHROOMS; AGARICUS-BLAZEI;
D O I：
10.1615/IntJMedMushr.v14.i4.90
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method of detecting carbohydrates (fucose, trehalose, mannitol, arabitol, mannose, glucose, galactose, fructose, and ribose) by high-performance anion chromatography pulsed amperometric detection (HAPEC-PAD) was established. The conditions are: CarboPac MA1 column, NaOH as the eluent, temperature 30 degrees C, Au working electrode, Ag/AgCl reference electrode, and flow rate 0.4 mL/min. These nine analytes, which yielded high resolution by this method, could be detected in 40 minutes. Mushrooms were tested and good precision, stability, and reproducibility were achieved. This method is suitable for mushroom samples and could support research and development on sugar and sugar alcohol, which contains special effects.
引用
收藏
页码：411 / 417
页数：7
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