Ethanolic Extract of Alternanthera sessilis (AS-1) Inhibits IgE-mediated Allergic Response in RBL-2H3 Cells

被引：7

作者：

Rayees, Sheikh ^{[1
]}

Kumar, Ajay ^{[1
]}

Rasool, Shafaq ^{[2
]}

Kaiser, Peerzada ^{[1
]}

Satti, Naresh Kumar ^{[3
]}

Sangwan, Payare Lal ^{[3
]}

Singh, Surjeet ^{[1
]}

Johri, Rakesh Kamal ^{[1
]}

Singh, Gurdarshan ^{[1
]}

机构：

[1] Indian Inst Integrat Med, CSIR, Div Pharmacol, Jammu 180001, India

[2] Shri Mata Vaishno Devi Univ, Sch Biotechnol, Katra Jammu, India

[3] Indian Inst Integrat Med, CSIR, Div Chem, Jammu 180001, India

来源：

IMMUNOLOGICAL INVESTIGATIONS | 2013年 / 42卷 / 06期

关键词：

Alternanthera sessilis; RBL-2H3; cells; IL-6; TNF-alpha; IL-13; IL-4; NF-kappa B; I kappa B alpha; beta-hexoaminidase; MAST-CELLS; CYTOKINE PRODUCTION; TNF-ALPHA; KAPPA-B; DEGRANULATION; RELEASE;

D O I：

10.3109/08820139.2013.789909

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The present study was designed to investigate the anti-allergic effects of ethanolic extract of Alternanthera sessilis (AS-1) in rat basophilic leukemia (RBL-2H3) cells. It significantly reduced the beta-hexosaminidase release from anti-DNP-IgE sensitized RBL-2H3 cells. AS-1also inhibited the IgE antibody-induced increase in Interleukin-6 (IL-6), TNF-alpha, IL-13 and IL-4 production in these cells. The inhibitory effect of AS-1 on these cytokine was found to be nuclear factor-KB (NF-kB) dependent, as it attenuated the degradation of IKBa and nuclear translocation of NFkB. In addition, AS-1 significantly attenuated the DNP HAS-induced intracellular Ca2+ release from these cells, which makes us speculate strongly that the decreased intracellular Ca2+ is involved in the inhibitory effect of AS-1 on beta-hexoaminidase release. Taken together, anti-allergic effects of AS-1 suggest possible therapeutic application of this extract in allergic diseases.

引用

页码：470 / 480

页数：11

共 33 条

[1]

AGRAWAL YK, 1978, POL J CHEM, V52, P2495

[2] Correlation between cytosolic calcium concentration and degranulation in RBL-2H3 cells in the presence of various concentrations of antigen-specific IgEs [J].

Aketani, S ;

Teshima, R ;

Umezawa, Y ;

Sawada, J .

IMMUNOLOGY LETTERS, 2001, 75 (03) :185-189

[3] Substance P induces TNF-α and IL-6 production through NFκB in peritoneal mast cells [J].

Azzolina, A ;

Bongiovanni, A ;

Lampiasi, N .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2003, 1643 (1-3) :75-83

[4]

BEAVEN MA, 1984, J BIOL CHEM, V259, P7129

[5] The allergic cascade: Review of the most important molecules in the asthmatic lung [J].

Bloemen, Karollen ;

Verstraelen, Sandra ;

Van Den Heuvel, Rosette ;

Witters, Hilda ;

Nelissen, Inge ;

Schoeters, Greet .

IMMUNOLOGY LETTERS, 2007, 113 (01) :6-18

[6]

Caro AA, 2001, MOL PHARMACOL, V60, P742

[7]

DOGRA J V V, 1977, Plant Biochemical Journal, V4, P14

[8] Role of phosphoinositide 3-kinase signaling in mast cells: new insights from knockout mouse studies [J].

Fukao, T ;

Terauchi, Y ;

Kadowaki, T ;

Koyasu, S .

JOURNAL OF MOLECULAR MEDICINE-JMM, 2003, 81 (09) :524-535

[9] CYTOKINE PRODUCTION BY MAST-CELLS AND BASOPHILS [J].

GALLI, SJ ;

GORDON, JR ;

WERSHIL, BK .

CURRENT OPINION IN IMMUNOLOGY, 1991, 3 (06) :865-873

[10] Role of Ca2+ on TNF-α and IL-6 secretion from RBL-2H3 mast cells [J].

Jeong, HJ ;

Hong, SH ;

Lee, DJ ;

Park, JH ;

Kim, KS ;

Kim, HM .

CELLULAR SIGNALLING, 2002, 14 (07) :633-639

← 1 2 3 4 →