Deficiency of liver-derived insulin-like growth factor-I (IGF-I) does not interfere with the skin wound healing rate

被引：18

作者：

Botusan, Ileana Ruxandra ^{[1
,2
,3
]}

Zheng, Xiaowei ^{[1
,2
]}

Narayanan, Sampath ^{[1
]}

Grunler, Jacob ^{[1
]}

Sunkari, Vivekananda Gupta ^{[1
]}

Calissendorff, Freja S. ^{[1
]}

Ansurudeen, Ishrath ^{[1
]}

Illies, Christopher ^{[4
]}

Svensson, Johan ^{[5
]}

Jansson, John-Olov ^{[6
]}

Ohlsson, Claes ^{[5
]}

Brismar, Kerstin ^{[1
,2
]}

Catrina, Sergiu-Bogdan ^{[1
,2
,3
]}

机构：

[1] Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden

[2] Karolinska Univ Hosp, Karolinska Inst, Dept Endocrinol Diabet & Metab, Stockholm, Sweden

[3] Stockholm Cty Council, Acad Specialist Ctr, Ctr Diabet, Stockholm, Sweden

[4] Karolinska Univ Hosp, Karolinska Inst, Dept Clin Pathol, Stockholm, Sweden

[5] Univ Gothenburg, Sahlgrenska Acad, Inst Internal Med, Gothenburg, Sweden

[6] Univ Gothenburg, Sahlgrenska Acad, Inst Neurosci & Physiol, Gothenburg, Sweden

来源：

PLOS ONE | 2018年 / 13卷 / 03期

基金：

瑞典研究理事会;

关键词：

TISSUE CONCENTRATIONS; DIABETIC-PATIENTS; HORMONE; SYSTEM; GENE; MICE; ABNORMALITIES; MOUSE; ACID;

D O I：

10.1371/journal.pone.0193084

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Objective IGF-I is a growth factor, which is expressed in virtually all tissues. The circulating IGF-I is however derived mainly from the liver. IGF-I promotes wound healing and its levels are decreased in wounds with low regenerative potential such as diabetic wounds. However, the contribution of circulating IGF-I to wound healing is unknown. Here we investigated the role of systemic IGF-I on wound healing rate in mice with deficiency of liver-derived IGF-I (LI-IGF-I-/- mice) during normal (normoglycemic) and impaired wound healing (diabetes). Methods LI-IGF-I-/- mice with complete inactivation of the IGF-I gene in the hepatocytes were generated using the Cre/loxP recombination system. This resulted in a 75% reduction of circulating IGF-I. Diabetes was induced with streptozocin in both LI-IGF-I-/- and control mice. Wounds were made on the dorsum of the mice, and the wound healing rate and histology were evaluated. Serum IGF-I and GH were measured by RIA and ELISA respectively. The expression of IGF-I, IGF-II and the IGF-I receptor in the skin were evaluated by qRT-PCR. The local IGF-I protein expression in different cell types of the wounds during wound healing process was analyzed using immunohistochemistry. Results The wound healing rate was similar in LI-IGF-I-/- mice to that in controls. Diabetes significantly delayed the wound healing rate in both LI-IGF-I-/- and control mice. However, no significant difference was observed between diabetic animals with normal or reduced hepatic IGF-I production. The gene expression of IGF-I, IGF-II and IGF-I receptor in skin was not different between any group of animals tested. Local IGF-I levels in the wounds were similar between of LI-IGF-I-/- and WT mice although a transient reduction of IGF-I expression in leukocytes in the wounds of LI-IGF-I-/- was observed seven days post wounding. Conclusion Deficiency in the liver-derived IGF-I does not affect wound healing in mice, neither in normoglycemic conditions nor in diabetes.

引用

页数：11

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