Inhibition of autophagy induces IL-1β release from ARPE-19 cells via ROS mediated NLRP3 inflammasome activation under high glucose stress

被引：118

作者：

Shi, Huanqi ^{[1
]}

Zhang, Zhen ^{[1
]}

Wang, Xiaodan ^{[1
]}

Li, Ruishu ^{[1
]}

Hou, Wenwen ^{[1
]}

Bi, Wenjiao ^{[1
]}

Zhang, Xiaomei ^{[1
]}

机构：

[1] Harbin Med Univ, Ophthalmol Hosp, Affiliated Hosp 1, Harbin 150001, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 463卷 / 04期

关键词：

Diabetic retinopathy; Autophagy; Retinal pigment epithelium; NLRP3; inflammasome;

D O I：

10.1016/j.bbrc.2015.06.060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Autophagy plays an important role in the development of diabetic retinopathy (DR). Retinal pigment epithelial (RPE) cells are the main cells involved in DR, a process in which hyperglycemia plays a crucial role. This study was conducted to investigate the protective effect of autophagy against high glucose-induced inflammatory response in ARPE-19 cells and its underlying mechanism. In the present study we subjected ARPE-19 cells to high glucose stress and showed that ARPE-19 cells respond to high glucose with an increase in autophagy. 3-methyladenine (3-MA) inhibited occurrence of autophagy and it leaded to the accumulation of damaged-mitochondria-producing-ROS, and the activation of NLRP3 inflammasome, and subsequently, caused IL-1 beta secretion. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：1071 / 1076

页数：6

共 24 条

[1]

[Anonymous], 2010, NATURE, DOI DOI 10.1038/NATURE08938

[2]

[Anonymous], J CLIN EXP OPHTHALMO

[3] Oxidative stress: A cause and therapeutic target of diabetic complications [J].

Araki, Eiichi ;

Nishikawa, Takeshi .

JOURNAL OF DIABETES INVESTIGATION, 2010, 1 (03) :90-96

[4] Diabetic retinopathy [J].

Cheung, Ning ;

Mitchell, Paul ;

Wong, Tien Yin .

LANCET, 2010, 376 (9735) :124-136

[5] Determination of vitreous interleukin-1 (IL-1) and tumour necrosis factor (TNF) levels in proliferative diabetic retinopathy [J].

Demircan, N. ;

Safran, B. G. ;

Soylu, M. ;

Ozcan, A. A. ;

Sizmaz, S. .

EYE, 2006, 20 (12) :1366-1369

[6] Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica [J].

Dostert, Catherine ;

Petrilli, Virginie ;

Van Bruggen, Robin ;

Steele, Chad ;

Mossman, Brooke T. ;

Tschopp, Jurg .

SCIENCE, 2008, 320 (5876) :674-677

[7] Diabetic retinopathy [J].

Fong, DS ;

Aiello, LP ;

Ferris, FL ;

Klein, R .

DIABETES CARE, 2004, 27 (10) :2540-2553

[8] A ubiquitin-like system mediates protein lipidation [J].

Ichimura, Y ;

Kirisako, T ;

Takao, T ;

Satomi, Y ;

Shimonishi, Y ;

Ishihara, N ;

Mizushima, N ;

Tanida, I ;

Kominami, E ;

Ohsumi, M ;

Noda, T ;

Ohsumi, Y .

NATURE, 2000, 408 (6811) :488-492

[9] Contributions of Inflammatory Processes to the Development of the Early Stages of Diabetic Retinopathy [J].

Kern, Timothy S. .

EXPERIMENTAL DIABETES RESEARCH, 2007, :95103

[10] Role of interleukin-1β in the pathogenesis of diabetic retinopathy [J].

Kowluru, RA ;

Odenbach, S .

BRITISH JOURNAL OF OPHTHALMOLOGY, 2004, 88 (10) :1343-1347

← 1 2 3 →