Plant-Derived Phenolics Inhibit the Accrual of Structurally Characterised Protein and Lipid Oxidative Modifications

被引:9
|
作者
Soler-Cantero, Arantza [1 ]
Jove, Mariona [2 ]
Cacabelos, Daniel [2 ]
Boada, Jordi [2 ]
Naudi, Alba [2 ]
Romero, Maria-Paz [1 ]
Cassanye, Anna [2 ]
Serrano, Jose C. E. [2 ]
Arola, Lluis [3 ]
Valls, Josep [4 ]
Josep Bellmunt, Maria [2 ]
Prat, Joan [2 ]
Pamplona, Reinald [2 ]
Portero-Otin, Manuel [2 ]
Motilva, Maria-Jose [1 ]
机构
[1] Univ Lleida, Escola Tecn Super Engn Agraria, Dept Food Technol, CeRTA TPV, Lleida, Spain
[2] Lleida Univ, Lleida IRBLLEIDA, Dept Expt Med, NUTREN Nutrigen Ctr, Lleida, Spain
[3] Univ Rovira & Virgili, Nutrigen Res Grp, Dept Biochem & Biotechnol, Tarragona, Spain
[4] Shirota Funct Foods, SL, Reus, Spain
来源
PLOS ONE | 2012年 / 7卷 / 08期
关键词
LOW-DENSITY-LIPOPROTEIN; HEPATOMA HEPG2 CELLS; OLIVE OIL PHENOLS; ANTIOXIDANT ACTIVITY; GALLIC ACID; ALPHA-TOCOPHEROL; RED WINE; LDL; PEROXIDATION; SUPPLEMENTATION;
D O I
10.1371/journal.pone.0043308
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Epidemiological data suggest that plant-derived phenolics beneficial effects include an inhibition of LDL oxidation. After applying a screening method based on 2,4-dinitrophenyl hydrazine-protein carbonyl reaction to 21 different plant-derived phenolic acids, we selected the most antioxidant ones. Their effect was assessed in 5 different oxidation systems, as well as in other model proteins. Mass-spectrometry was then used, evidencing a heterogeneous effect on the accumulation of the structurally characterized protein carbonyl glutamic and aminoadipic semialdehydes as well as for malondialdehyde-lysine in LDL apoprotein. After TOF based lipidomics, we identified the most abundant differential lipids in Cu++-incubated LDL as 1-palmitoyllysophosphatidylcholine and 1-stearoyl-sn-glycero-3-phosphocholine. Most of selected phenolic compounds prevented the accumulation of those phospholipids and the cellular impairment induced by oxidized LDL. Finally, to validate these effects in vivo, we evaluated the effect of the intake of a phenolic-enriched extract in plasma protein and lipid modifications in a well-established model of atherosclerosis (diet-induced hypercholesterolemia in hamsters). This showed that a dietary supplement with a phenolic-enriched extract diminished plasma protein oxidative and lipid damage. Globally, these data show structural basis of antioxidant properties of plant-derived phenolic acids in protein oxidation that may be relevant for the health-promoting effects of its dietary intake.
引用
收藏
页数:12
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