Metallo-regulation of the bimolecular triplex formation of a peptide nucleic acid

被引：9

作者：

Shimada, Hiroshi ^{[1
]}

Sakurai, Toshihiko ^{[2
]}

Kitamura, Yusuke ^{[1
,3
]}

Matsuura, Hirotaka ^{[1
]}

Ihara, Toshihiro ^{[1
,3
]}

机构：

[1] Kumamoto Univ, Grad Sch Sci & Technol, Dept Appl Chem & Biochem, Chuo Ku, Kumamoto 8608555, Japan

[2] Tottori Univ, Grad Sch Engn, Dept Chem & Biotechnol, Tottori 6808550, Japan

[3] Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Tokyo 1020076, Japan

来源：

DALTON TRANSACTIONS | 2013年 / 42卷 / 45期

关键词：

HELIX FORMATION; SOLID-PHASE; DNA; PNA; SEQUENCE; HYBRIDIZATION; BINDING; PROBES; THERMODYNAMICS; RECOGNITION;

D O I：

10.1039/c3dt51386f

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Peptide nucleic acid (PNA) conjugates incorporating a bipyridine unit were prepared. The bipyridine was built into the loop moiety of PNAs that were designed to specifically form a hairpin and a PNA/DNA bimolecular triplex. While the thermal stability of the hairpin structure was only minimally affected by Cu2+ addition, the PNA/DNA bimolecular triplex structure was significantly destabilized by complexation with Cu2+. The melting temperature of the bimolecular triplex decreased by 17.4 degrees C in the presence of Cu2+. This corresponds to more than a 1000 fold decrease in the binding constant for bimolecular triplex formation. Upon complexation, the bipyridine unit underwent a drastic conformational change which accounts for the observed differences in the thermal stabilities of the triplex upon binding. The bipyridine- PNA conjugate may be useful as an allosteric DNA carrier that releases the DNA in response to a certain metal ion concentration.

引用

页码：16006 / 16013

页数：8

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