Killing by Bactericidal Antibiotics Does Not Depend on Reactive Oxygen Species

被引：405

作者：

Keren, Iris ^{[1
]}

Wu, Yanxia ^{[1
]}

Inocencio, Julio ^{[1
]}

Mulcahy, Lawrence R. ^{[1
]}

Lewis, Kim ^{[1
]}

机构：

[1] Northeastern Univ, Dept Biol, Antimicrobial Discovery Ctr, Boston, MA 02115 USA

来源：

SCIENCE | 2013年 / 339卷 / 6124期

关键词：

ESCHERICHIA-COLI; PERSISTER CELLS; DEATH; TOLERANCE; RESISTANCE; LETHALITY; MECHANISM; LYSIS;

D O I：

10.1126/science.1232688

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bactericidal antibiotics kill by modulating their respective targets. This traditional view has been challenged by studies that propose an alternative, unified mechanism of killing, whereby toxic reactive oxygen species (ROS) are produced in the presence of antibiotics. We found no correlation between an individual cell's probability of survival in the presence of antibiotic and its level of ROS. An ROS quencher, thiourea, protected cells from antibiotics present at low concentrations, but the effect was observed under anaerobic conditions as well. There was essentially no difference in survival of bacteria treated with various antibiotics under aerobic or anaerobic conditions. This suggests that ROS do not play a role in killing of bacterial pathogens by antibiotics.

引用

页码：1213 / 1216

页数：4

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