Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter

被引：276
作者：
Donovan, RS
Robinson, CW
Glick, BR
机构：
[1] UNIV WATERLOO, DEPT BIOL, WATERLOO, ON N2L 3G1, CANADA
[2] UNIV WATERLOO, DEPT CHEM ENGN, WATERLOO, ON N2L 3G1, CANADA
来源：
JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1996年 / 16卷 / 03期
关键词：
lac promoter; tac promoter; recombinant DNA; protein overexpression; fermentation strategies; IPTG; lactose;
D O I：
10.1007/BF01569997
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This review examines factors which influence the expression of foreign proteins in Escherichia coli under the transcriptional control of the lac and tao promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Specifically, the influence of IPTG (isopropyl-beta-o-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed.
引用
收藏
页码：145 / 154
页数：10
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