Identification and phylogenetic analysis of a sheep pox virus isolated from the Ningxia Hui Autonomous Region of China

被引：15
作者：
Zhu, X. L. [1 ]
Yang, F. [1 ]
Li, H. X. [1 ]
Dou, Y. X. [1 ]
Meng, X. L. [1 ]
Li, H. [1 ]
Luo, X. N. [1 ]
Cai, X. P. [1 ]
机构：
[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Engn Res Ctr Biol Detect Gansu Prov, Natl Foot & Mouth Dis Reference Lab,State Key Lab, Lanzhou, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2013年 / 12卷 / 02期
关键词：
Capripoxvirus; Small-tailed Han sheep; ORF; 095; gene; 103; P32; Phylogenetic analysis; POXVIRUS IDENTIFICATION; PCR; CAPRIPOXVIRUS; DIFFERENTIATION; OUTBREAK; PROVINCE; GOATS;
D O I：
10.4238/2013.May.14.7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An outbreak of sheep pox was investigated in the Ningxia Hui Autonomous Region in China. Through immunofluorescence testing, isolated viruses, polymerase chain reaction identification, and electron microscopic examination, the isolated strain was identified as a sheep pox virus. The virus was identified through sequence and phylogenetic analysis of the P32 gene, open reading frame (ORF) 095, and ORF 103 genes. This study is the first to use the ORF 095 and ORF 103 genes as candidate genes for the analysis of sheep pox. The results showed that the ORF 095 and ORF 103 genes could be used for the genotyping of the sheep pox virus.
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收藏
页码：1670 / 1678
页数：9
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