Development of a novel smartphone-based application for accurate and sensitive on-field hemoglobin measurement

被引：11

作者：

Ghatpande, Niraj S. ^{[1
]}

Apte, Priti P. ^{[1
]}

Joshi, Bimba N. ^{[1
]}

Naik, Sadanand S. ^{[2
]}

Bodas, Dhananjay ^{[3
]}

Sande, Vishwajit ^{[4
]}

Uttarwar, Pravin ^{[4
]}

Kulkarni, Prasad P. ^{[1
]}

机构：

[1] Agharkar Res Inst, Bioprospecting Grp, GG Agarkar Rd, Pune 411004, Maharashtra, India

[2] KEM Hosp & Res Ctr, Pune 411011, Maharashtra, India

[3] Agharkar Res Inst, Nanobiosci Grp, GG Agarkar Rd, Pune 411004, Maharashtra, India

[4] Mindbowser Infosolut Pvt Ltd, Gwalior 474012, India

来源：

RSC ADVANCES | 2016年 / 6卷 / 106期

关键词：

RESOURCE-LIMITED SETTINGS; PAPER; PHONE; DIAGNOSIS;

D O I：

10.1039/c6ra24366e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here, we report on the development of a smartphone-based application (app) for the accurate and sensitive measurement of hemoglobin (Hb). Hb values obtained using the app correlated significantly (R-2 = 0.976) with those obtained by an automated hematology analyzer. Bland-Altman analysis showed that the standard deviation of the difference between Hb measured by the two methods was 0.35 g dL(-1) and the limits of agreement between the two methods were -0.62 to 0.75. A limit of detection of 0.66 g dL(-1) was achieved using the app along with a 2 g dL(-1) limit of quantification. Furthermore, measurement using the app was highly sensitive (94%) and specific (90%). The app was developed for a smartphone and it will be particularly useful in resource-limited conditions for the rapid, accurate, highly sensitive and specific assessment of anemia.

引用

页码：104067 / 104072

页数：6

共 25 条

[1] Comparison of HemoCue® hemoglobin-meter and automated hematology analyzer in measurement of hemoglobin levels in pregnant women at Khartoum hospital, Sudan [J].

Adam, Ishag ;

Ahmed, Samah ;

Mahmoud, Mahmoud H. ;

Yassin, Mohammed I. .

DIAGNOSTIC PATHOLOGY, 2012, 7

[2]

[Anonymous], 2015, Ericsson Mobility Report

[3]

[Anonymous], 2000, Fed Regist, V65, P776

[4] STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT [J].

BLAND, JM ;

ALTMAN, DG .

LANCET, 1986, 1 (8476) :307-310

[5] Chromatography paper as a low-cost medium for accurate spectrophotometric assessment of blood hemoglobin concentration [J].

Bond, Meaghan ;

Elguea, Carlos ;

Yan, Jasper S. ;

Pawlowski, Michal ;

Williams, Jessica ;

Wahed, Amer ;

Oden, Maria ;

Tkaczyk, Tomasz S. ;

Richards-Kortum, Rebecca .

LAB ON A CHIP, 2013, 13 (12) :2381-2388

[6] Mobile Device for Disease Diagnosis and Data Tracking in Resource-Limited Settings [J].

Chin, Curtis D. ;

Cheung, Yuk Kee ;

Laksanasopin, Tassaneewan ;

Modena, Mario M. ;

Chin, Sau Yin ;

Sridhara, Archana A. ;

Steinmiller, David ;

Linder, Vincent ;

Mushingantahe, Jules ;

Umviligihozo, Gisele ;

Karita, Etienne ;

Mwambarangwe, Lambert ;

Braunstein, Sarah L. ;

van de Wijgert, Janneke ;

Sahabo, Ruben ;

Justman, Jessica E. ;

El-Sadr, Wafaa ;

Sia, Samuel K. .

CLINICAL CHEMISTRY, 2013, 59 (04) :629-640

[7] Validation of the WHO Hemoglobin Color Scale Method [J].

Darshana, Leeniyagala Gamaralalage Thamal ;

Uluwaduge, Deepthi Inoka .

ANEMIA, 2014, 2014

[8] Use of a mobile phone for potentiostatic control with low cost paper-based microfluidic sensors [J].

Delaney, Jacqui L. ;

Doeven, Egan H. ;

Harsant, Anthony J. ;

Hogan, Conor F. .

ANALYTICA CHIMICA ACTA, 2013, 790 :56-60

[9] Smartphone dongle for simultaneous measurement of hemoglobin concentration and detection of HIV antibodies [J].

Guo, Tiffany ;

Patnaik, Ritish ;

Kuhlmann, Kevin ;

Rai, Alex J. ;

Sia, Samuel K. .

LAB ON A CHIP, 2015, 15 (17) :3514-3520

[10] A perspective on paper-based microfluidics: Current status and future trends [J].

Li, Xu ;

Ballerini, David R. ;

Shen, Wei .

BIOMICROFLUIDICS, 2012, 6 (01)

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