Extraction of High-Quality RNA from Rubber Tree Leaves

被引：14

作者：

Deng, Liu-Hong ^{[2
]}

Luo, Ming-Wu ^{[1
]}

Zhang, Chun-Fa ^{[2
]}

Zeng, Hui-Cai ^{[2
]}

机构：

[1] Hainan Univ, Coll Mat Sci & Chem Engn, Haikou 570228, Peoples R China

[2] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2012年 / 76卷 / 07期

基金：

海南省自然科学基金; 中国国家自然科学基金;

关键词：

rubber-tree leaves; RNA extraction; cetyltrimethylammonium bromide (CTAB); TRIzol; rapid amplification of cDNA ends (RACE);

D O I：

10.1271/bbb.120014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE.

引用

页码：1394 / 1396

页数：3

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