Development and characterization of twelve microsatellite markers for miiuy croaker

Twelve microsatellite DNA markers were reported for miiuy croaker in this paper, of which nine were developed by 5'anchored PCR technique, and three were detected in three genes (CC, cathepsin D and CCL4) of miiuy croaker. A total of 63 alleles were identified in 30 individuals and the number of alleles per locus ranged from three to eight, with an average of 5.3. The observed and expected heterozygosity per locus ranged from 0.4667 to 1.0000 and from 0.4910 to 0.7755, respectively. Significant deviation from Hardy-Weinberg equilibrium was detected at two microsatellite loci after Bonferroni correction (P < 0.0042). No significant linkage disequilibrium was found between all pairs of twelve loci. These loci will be helpful for better understanding of genetic background of miiuy croaker.