Mutagenesis and phenotyping resources in zebrafish for studying development and human disease

被引：37

作者：

Varshney, Gaurav Kumar ^{[1
]}

Burgess, Shawn Michael ^{[1
]}

机构：

[1] NHGRI, Genome Technol Branch, Bethesda, MD 20892 USA

来源：

BRIEFINGS IN FUNCTIONAL GENOMICS | 2014年 / 13卷 / 02期

基金：

美国国家卫生研究院;

关键词：

zebrafish; mutagenesis; phenotyping; resources; knockouts; TARGETED GENE DISRUPTION; ZINC-FINGER NUCLEASES; INSERTIONAL MUTAGENESIS; GENOME-WIDE; EFFICIENT CONSTRUCTION; CHROMOSOMAL DELETIONS; POSITIONAL CLONING; POINT MUTATIONS; MODEL ORGANISM; TAL EFFECTORS;

D O I：

10.1093/bfgp/elt042

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The zebrafish (Danio rerio) is an important model organism for studying development and human disease. The zebrafish has an excellent reference genome and the functions of hundreds of genes have been tested using both forward and reverse genetic approaches. Recent years have seen an increasing number of large-scale mutagenesis projects and the number of mutants or gene knockouts in zebrafish has increased rapidly, including for the first time conditional knockout technologies. In addition, targeted mutagenesis techniques such as zinc finger nucleases, transcription activator-like effector nucleases and clustered regularly interspaced short sequences (CRISPR) or CRISPR-associated (Cas), have all been shown to effectively target zebrafish genes as well as the first reported germline homologous recombination, further expanding the utility and power of zebrafish genetics. Given this explosion of mutagenesis resources, it is now possible to perform systematic, high-throughput phenotype analysis of all zebrafish gene knockouts.

引用

页码：82 / 94

页数：13

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