The Influence of Matrix Degradation and Functionality on Cell Survival and Morphogenesis in PEG-Based Hydrogels

被引：42
作者：
Raza, Asad [1 ]
Lin, Chien-Chi [1 ]
机构：
[1] Indiana Univ Purdue Univ, Dept Biomed Engn, Purdue Sch Engn & Technol, Indianapolis, IN 46202 USA
来源：
MACROMOLECULAR BIOSCIENCE | 2013年 / 13卷 / 08期
关键词：
cell; material interactions; degradation; hydrogels; mesenchymal stem cells; POLY(ETHYLENE GLYCOL) HYDROGELS; MESENCHYMAL STEM-CELLS; OSTEOGENIC DIFFERENTIATION; IN-VITRO; CHONDROGENIC DIFFERENTIATION; PEPTIDE; MODULUS; BIOMATERIALS; VIABILITY; CHEMISTRY;
D O I：
10.1002/mabi.201300044
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two norbornene-functionalized PEG macromers are synthesized to render hydrogels with different hydrolytic degradability. Dithiol-containing linkers such as dithiothreitol or biscysteine-containing peptides are used to control proteolytic degradability. The influence of thiol-ene gel degradability on cell survival and morphogenesis in 3D is assessed using hMSCs and pancreatic MIN6 cells. The initial cell viability can be negatively affected in highly crosslinked thiol-ene hydrogels. When cells are encapsulated in thiol-ene gels lacking cell-adhesive motifs, their survival and proliferation are promoted in more hydrolytically labile hydrogels. The degree of 3D cell spreading in encapsulated hMSCs is enhanced when the matrices are immobilized with cell-adhesive motifs.
引用
收藏
页码：1048 / 1058
页数：11
相关论文
共 50 条
  • [1] The performance of human mesenchymal stem cells encapsulated in cell-degradable polymer-peptide hydrogels
    Anderson, Sarah B.
    Lin, Chien-Chi
    Kuntzler, Donna V.
    Anseth, Kristi S.
    [J]. BIOMATERIALS, 2011, 32 (14) : 3564 - 3574
  • [2] In situ forming degradable networks and their application in tissue engineering and drug delivery
    Anseth, KS
    Metters, AT
    Bryant, SJ
    Martens, PJ
    Elisseeff, JH
    Bowman, CN
    [J]. JOURNAL OF CONTROLLED RELEASE, 2002, 78 (1-3) : 199 - 209
  • [3] VISIBLE LIGHT PHOTOINITIATION OF MESENCHYMAL STEM CELL-LADEN BIORESPONSIVE HYDROGELS
    Bahney, C. S.
    Lujan, T. J.
    Hsu, C. W.
    Bottlang, M.
    West, J. L.
    Johnstone, B.
    [J]. EUROPEAN CELLS & MATERIALS, 2011, 22 : 43 - 55
  • [4] A bioresponsive hydrogel tuned to chondrogenesis of human mesenchymal stem cells
    Bahney, Chelsea S.
    Hsu, Chih-Wei
    Yoo, Jung U.
    West, Jennifer L.
    Johnstone, Brian
    [J]. FASEB JOURNAL, 2011, 25 (05) : 1486 - 1496
  • [5] The effect of heparin-functionalized PEG hydrogels on three-dimensional human mesenchymal stem cell osteogenic differentiation
    Benoit, Danielle S. W.
    Durney, Andrew R.
    Anseth, Krish S.
    [J]. BIOMATERIALS, 2007, 28 (01) : 66 - 77
  • [6] Compositional control of poly(ethylene glycol) hydrogel modulus independent of mesh size
    Browning, M. B.
    Wilems, T.
    Hahn, M.
    Cosgriff-Hernandez, E.
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2011, 98A (02) : 268 - 273
  • [7] Development of a Biostable Replacement for PEGDA Hydrogels
    Browning, Mary Beth
    Cosgriff-Hernandez, Elizabeth
    [J]. BIOMACROMOLECULES, 2012, 13 (03) : 779 - 786
  • [8] Encapsulating Chondrocytes in degrading PEG hydrogels with high modulus: Engineering gel structural changes to facilitate cartilaginous tissue production
    Bryant, SJ
    Bender, RJ
    Durand, KL
    Anseth, KS
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 2004, 86 (07) : 747 - 755
  • [9] Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering
    Burdick, JA
    Anseth, KS
    [J]. BIOMATERIALS, 2002, 23 (22) : 4315 - 4323
  • [10] Suppression of the Epidermal Growth Factor Receptor Inhibits Epithelial-Mesenchymal Transition in Human Pancreatic Cancer PANC-1 Cells
    Chang, Zhi-Gang
    Wei, Jun-Min
    Qin, Chang-Fu
    Hao, Kun
    Tian, Xiao-Dong
    Xie, Kun
    Xie, Xue-Hai
    Yang, Yin-Mo
    [J]. DIGESTIVE DISEASES AND SCIENCES, 2012, 57 (05) : 1181 - 1189
12345