Production of isomalto-oligosaccharides using dextransucrase immobilized in alginate fibres

被引：40

作者：

Tanriseven, A ^{[1
]}

Dogan, S ^{[1
]}

机构：

[1] Gebze Inst Technol, Dept Biochem, Gebze, Kocaeli, Turkey

来源：

PROCESS BIOCHEMISTRY | 2002年 / 37卷 / 10期

关键词：

alginate fibre; dextransucrase; immobilization; isomalto-oligosaccharides;

D O I：

10.1016/S0032-9592(01)00319-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isomalto-oligosaccharides were produced using Leuconostoc mesenteroides B-512 FM dextransucrase immobilized in alginate fibres. Immobilization resulted in 90% relative activity. The immobilized enzyme retained activity for ten batch reactions without a decrease in activity and was reacted with a substrate composed of sucrose (1 M) and glucose (1 M) to obtain isomalto-oligosaccharides. Immobilization of dextransucrase in alginate fibre is superior to that in alginate beads in terms of immobilization yield and repetitive use. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1111 / 1115

页数：5

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