Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination

被引：37

作者：

Isobe, Keisuke ^{[1
,2
]}

Takeda, Takanori ^{[1
,3
]}

Mochizuki, Kyohei ^{[1
,3
]}

Song, Qiyuan ^{[2
,4
]}

Suda, Akira ^{[3
]}

Kannari, Fumihiko ^{[4
]}

Kawano, Hiroyuki ^{[5
]}

Kumagai, Akiko ^{[5
]}

Miyawaki, Atsushi ^{[2
,5
]}

Midorikawa, Katsumi ^{[1
,2
]}

机构：

[1] RIKEN, Laser Technol Lab, Wako, Saitama 3510198, Japan

[2] RIKEN Ctr Adv Photon, Wako, Saitama 3510198, Japan

[3] Tokyo Univ Sci, Grad Sch Sci & Technol, Dept Phys, Noda, Chiba 2788510, Japan

[4] Keio Univ, Dept Elect & Elect Engn, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

[5] RIKEN Brain Sci Inst, Lab Cell Funct Dynam, Wako, Saitama 3510198, Japan

来源：

BIOMEDICAL OPTICS EXPRESS | 2013年 / 4卷 / 11期

关键词：

PATTERNED EXCITATION MICROSCOPY; IMAGING-DEPTH LIMIT; MULTIPHOTON MICROSCOPY; WIDEFIELD MULTIPHOTON; THICK TISSUE; LIGHT;

D O I：

10.1364/BOE.4.002396

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate super-resolution imaging with background fluorescence rejection by interferometric temporal focusing microscopy, in which temporal focusing is combined with structured illumination. The lateral resolution and the optical sectioning capability are simultaneously improved by factors of 1.6 and 1.4, respectively, compared to conventional temporal focusing microscopy. Fluorescent beads (200 nm diameter) that are difficult to distinguish from the background fluorescence in conventional temporal focusing microscopy, are clearly visualized by interferometric temporal focusing microscopy. (C) 2013 Optical Society of America

引用

页码：2396 / 2410

页数：15

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