Production of three O-methhylated esculetins with Escherichia coli expressing O-methyltransferase from poplar

被引：20
作者：
Kim, Bong Gyu
Lee, Youngshim
Hur, Hor-Gil
Lim, Yoongho
Ahn, Joong-Hoon [1 ]
机构：
[1] Konkuk Univ, Biomol Informat Ctr, Dept Biosci & Biotechnol, Seoul 143701, South Korea
[2] Gwangju Inst Sci & Technol, Dept Environm Sci & Engn, Kwangju, South Korea
[3] Gwangju Inst Sci & Technol, Int Environm Res Ctr, Kwangju, South Korea
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 05期
关键词：
biotransformation; esculetin; O-methyltransferase;
D O I：
10.1271/bbb.70.1269
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
O-Methyltransferase, POMT-9 was expressed in Escherichia coli. HPLC analysis of reaction products revealed three peaks corresponding to isoscopoletin, scopoletin, and scoparone, and their structures were determined using NMR. Biotransformation of esculetin with E. colt expressing POMT-9 generated scopoletin, isoscopoletin, and scoparone at 30.3, 21, and 31 Km respectively. POMT-9 is the first O-methyltransferase that produces three different O-methylated products.
引用
收藏
页码：1269 / 1272
页数：4
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