Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan

被引:26
作者
Yano, Takahisa [1 ,2 ]
Yamagami, Ryoko [1 ,2 ]
Misumi, Kazuhiro
Kubota, Chikara [3 ]
Moe, Kyaw Kyaw [1 ,2 ]
Hayashi, Tetsuya [4 ,5 ,8 ]
Yoshitani, Kazunori [6 ]
Ohtake, Osamu [7 ]
Misawa, Naoaki [1 ,2 ,8 ]
机构
[1] Miyazaki Univ, Fac Agr, Dept Vet Sci, Lab Vet Publ Hlth, Miyazaki 8892192, Japan
[2] Kagoshima Univ, Fac Agr, Dept Vet Sci, Lab Vet Surg, Kagoshima 8900065, Japan
[3] Kagoshima Univ, Fac Agr, Dept Vet Sci, Lab Vet Reprod, Kagoshima 8900065, Japan
[4] Miyazaki Univ, Fac Med, Dept Infect Dis, Div Microbiol, Miyazaki 8891692, Japan
[5] Miyazaki Univ, Frontier Sci Res Ctr, Div Bioenvironm Sci, Miyazaki 8891692, Japan
[6] NOSAI Chiba, Hokubu Vet Clin Ctr, Chiba 2890407, Japan
[7] NOSAI Okayama, Tsuyama Vet Clin Ctr, Okayama 7080843, Japan
[8] Miyazaki Univ, Project Zoonoses Educ & Res, Miyazaki 8892192, Japan
关键词
INTERDIGITAL PAPILLOMATOSIS; GUGGENHEIMELLA-BOVIS; LESIONS; DIVERSITY; NOV;
D O I
10.1128/JCM.01574-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Papillomatous digital dermatitis (PDD) is an infectious foot disease of cattle that is prevalent throughout the world. Although it has been prevalent in Japan since the first case was reported in 1992, full epidemiological and bacteriological examinations have not been conducted. We collected 91 lesions of PDD from 80 dairy cattle on 12 farms in eight regions of Japan to isolate the spirochetes that are frequently detected in lesions. We isolated 40 strains of spirochetes from 24 cattle (30.0%) by a simple two-step culture technique, in which the biopsy samples were incubated at 4 C for 48 to 72 h in an enrichment broth supplemented with antibiotics, which improved the rate of isolation, and then inoculated on selective agar plates. All spirochetes examined were catalase positive and oxidase negative and showed weak beta-hemolytic activity. Enzyme activities were identical to those of Treponema phagedenis ATCC 27087. Sequencing of the 16S rRNA gene showed that all strains isolated had > 99% identity to those of the T. phagedenis type strain and of T. phagedenis-like strains isolated from PDD lesions in the United States and Europe. Pulsed-field gel electrophoresis and PCR-based random amplified polymorphism DNA methods revealed considerable diversity among strains isolated not only from different cattle but also from the same individuals. These findings may provide further evidence for the role of these treponemes in the pathogenesis of persistent PDD.
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收藏
页码:727 / 733
页数:7
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