The Antiestrogens Tamoxifen and Fulvestrant Abolish Estrogenic Impacts of 17α-ethinylestradiol on Male Calling Behavior of Xenopus laevis

被引:17
作者
Hoffmann, Frauke [1 ]
Kloas, Werner [1 ,2 ]
机构
[1] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Ecophysiol & Aquaculture, Berlin, Germany
[2] Humboldt Univ, Inst Biol, Dept Endocrinol, D-10099 Berlin, Germany
关键词
ENDOCRINE-DISRUPTING COMPOUNDS; AFFECTING REPRODUCTIVE-BIOLOGY; AFRICAN CLAWED FROGS; STICKLEBACK GASTEROSTEUS-ACULEATUS; MALE 3-SPINED STICKLEBACK; MESSENGER-RNA EXPRESSION; MALE SEX BEHAVIOR; BREAST-CANCER; ENVIRONMENTAL CHEMICALS; POMATOSCHISTUS-MINUTUS;
D O I
10.1371/journal.pone.0044715
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Various synthetic chemicals released to the environment can interfere with the endocrine system of vertebrates. Many of these endocrine disrupting compounds (EDCs) exhibit estrogenic activity and can interfere with sexual development and reproductive physiology. More recently, also chemicals with different modes of action (MOAs), such as antiestrogenic, androgenic and antiandrogenic EDCs, have been shown to be present in the environment. However, to date EDC-research primarily focuses on exposure to EDCs with just one MOA, while studies examining the effects of simultaneous exposure to EDCs with different MOAs are rare, although they would reflect more real, natural exposure situations. In the present study the combined effects of estrogenic and antiestrogenic EDCs were assessed by analyzing the calling behavior of short-term exposed male Xenopus laevis. The estrogenic 17 alpha-ethinylestradiol (EE2), and the antiestrogenic EDCs tamoxifen (TAM) and fulvestrant (ICI) were used as model substances. As previously demonstrated, sole EE2 exposure (10-10 M) resulted in significant alterations of the male calling behavior, including altered temporal and spectral parameters of the advertisement calls. Sole TAM (10-7 M, 10-8 M, 10-10 M) or ICI (10-7 M) exposure, on the other hand, did not affect any of the measured parameters. If frogs were co-exposed to EE2 (10-10 M) and TAM (10-7 M) the effects of EE2 on some parameters were abolished, but co-exposure to EE2 and ICI (10-7 M) neutralized all estrogenic effects. Thus, although EDCs with antiestrogenic MOA might not exhibit any effects per se, they can alter the estrogenic effects of EE2. Our observations demonstrate that there is need to further investigate the combined effects of EDCs with various, not only opposing, MOAs as this would reflect realistic wildlife situations.
引用
收藏
页数:8
相关论文
共 62 条
[1]   ANDROGEN CONTROL OF MALE SEX BEHAVIOR IN THE CRESTED NEWT (TRITURUS-CRISTATUS-CARNIFEX LAUR) - CASTRATION AND SEX STEROID-ADMINISTRATION [J].
ANDREOLETTI, GE ;
MALACARNE, G ;
VELLANO, C .
HORMONES AND BEHAVIOR, 1983, 17 (01) :103-110
[2]  
Bates D., 2009, Mixed-Effects Models in S and S-PLUS
[3]   Mate calling behavior of male South African clawed frogs (Xenopus laevis) is suppressed by the antiandrogenic endocrine disrupting compound flutamide [J].
Behrends, Thomas ;
Urbatzka, Ralph ;
Krackow, Sven ;
Elepfandt, Andreas ;
Kloas, Werner .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2010, 168 (02) :269-274
[4]   An endocrine disrupter increases growth and risky behavior in threespined stickleback (Gasterosteus aculeatus) [J].
Bell, AM .
HORMONES AND BEHAVIOR, 2004, 45 (02) :108-114
[5]   Effects of an endocrine disrupter on courtship and aggressive behaviour of male three-spined stickleback, Gasterosteus aculeatus [J].
Bell, AM .
ANIMAL BEHAVIOUR, 2001, 62 :775-780
[6]   Pharmaceuticals and Endocrine Disrupting Compounds in US Drinking Water [J].
Benotti, Mark J. ;
Trenholm, Rebecca A. ;
Vanderford, Brett J. ;
Holady, Janie C. ;
Stanford, Benjamin D. ;
Snyder, Shane A. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2009, 43 (03) :597-603
[7]   Molecular mechanism of action at estrogen receptor α of a new clinically relevant antiestrogen (GW7604) related to tamoxifen [J].
Bentrem, DJ ;
Dardes, RC ;
Liu, H ;
Maccgregor-Schafer, J ;
Zapf, JW ;
Jordan, VC .
ENDOCRINOLOGY, 2001, 142 (02) :838-846
[8]   Endocrine disrupting effects on the nesting behaviour of male three-spined stickleback Gasterosteus aculeatus L. [J].
Brian, J. V. ;
Augley, J. J. ;
Braithwaite, V. A. .
JOURNAL OF FISH BIOLOGY, 2006, 68 (06) :1883-1890
[9]   Endocrine disrupting chemicals (EDC) with (anti)estrogenic and (anti)androgenic modes of action affecting reproductive biology of Xenopus laevis:: II.: Effects on gonad histomorphology [J].
Cevasco, Alessandra ;
Urbatzka, Ralph ;
Bottero, Sergio ;
Massari, Alessandra ;
Pedemonte, Federico ;
Kloas, Wemer ;
Mandich, Alberta .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 2008, 147 (02) :241-251
[10]   Endocrine disrupters - potential modulators of the immune system and allergic response [J].
Chalubinski, M. ;
Kowalski, M. L. .
ALLERGY, 2006, 61 (11) :1326-1335