Development of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus

被引：0
|
作者：
Akrami, H. [1 ]
Hedayati, A. [2 ]
Farshian, M. [2 ]
Haqshenas, G. [3 ]
机构：
[1] Razi Univ, Dept Biol, Fac Sci, Kermanshah, Iran
[2] Iranian Vet Org, Tehran, Iran
[3] NRCGEB, Tehran, Iran
来源：
IRANIAN JOURNAL OF VETERINARY RESEARCH | 2013年 / 14卷 / 04期
关键词：
Infectious bronchitis virus; Dot-blot hybridization; Nucleocapsid protein;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, induces strong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blot test was developed to serologically examine chicken serum samples for the presence of IBV antibody. Initially, 72 serum samples were tested for the presence of IBV antibody using a commercial enzyme linked immunosorbent assay (ELISA) kit. Forty six IBV positive serum samples (group A) produced strong signals in the dot-blot assay. Seven negative serum samples (group B) produced weak but specific signals using the dot-blot assay in conjunction with Western blot analysis. The remaining 19 samples (group C) from IBV negative specific pathogen free (SPF) chickens did not produce visible signals using the dot-blot assay. In conclusion, the above results suggest that the dot-blot assay is a reliable, sensitive, and specific test for serological detection of IBV positive chickens.
引用
收藏
页码：350 / 353
页数：4
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