Evolution of vertebrate gill covers via shifts in an ancient Pou3f3 enhancer

被引：17

作者：

Barske, Lindsey ^{[1
,2
,3
]}

Fabian, Peter ^{[1
]}

Hirschberger, Christine ^{[4
]}

Jandzik, David ^{[5
,6
]}

Square, Tyler ^{[5
,7
]}

Xu, Pengfei ^{[1
]}

Nelson, Nellie ^{[1
]}

Yu, Haoze Vincent ^{[1
]}

Medeiros, Daniel M. ^{[5
]}

Gillis, J. Andrew ^{[4
,8
]}

Crump, J. Gage ^{[1
]}

机构：

[1] Univ Southern Calif, Dept Stem Cell Biol & Regenerat Med, Eli & Edythe Broad CIRM Ctr Regenerat Med & Stem, WM Keck Sch Med, Los Angeles, CA 90033 USA

[2] Univ Cincinnati, Coll Med, Dept Pediat, Cincinnati, OH 45229 USA

[3] Cincinnati Childrens Hosp Med Ctr, Div Human Genet, Cincinnati, OH 45229 USA

[4] Univ Cambridge, Dept Zool, Cambridge CB2 3EJ, England

[5] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA

[6] Comenius Univ, Dept Zool, Bratislava 84215, Slovakia

[7] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[8] Marine Biol Lab, Woods Hole, MA 02543 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2020年 / 117卷 / 40期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Pou3f3; gill cover; vertebrate evolution; neural crest; enhancer deletion; HOX EXPRESSION; SKELETAL; CARTILAGE; ARCH; PROTEINS; PATTERN; GROWTH;

D O I：

10.1073/pnas.2011531117

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Whereas the gill chambers of jawless vertebrates open directly into the environment, jawed vertebrates evolved skeletal append-ages that drive oxygenated water unidirectionally over the gills. A major anatomical difference between the two jawed vertebrate lineages is the presence of a single large gill cover in bony fishes versus separate covers for each gill chamber in cartilaginous fishes. Here, we find that these divergent patterns correlate with the pharyngeal arch expression of Pou3f3 orthologs. We identify a deeply conserved Pou3f3 arch enhancer present in humans through sharks but undetectable in jawless fish. Minor differences between the bony and cartilaginous fish enhancers account for their restricted versus pan-arch expression patterns. In zebrafish, mutation of Pou3f3 or the conserved enhancer disrupts gill cover formation, whereas ectopic pan-arch Pou3f3b expression generates ectopic skeletal elements resembling the multimeric covers of cartilaginous fishes. Emergence of this Pou3f3 arch enhancer >430 Mya and subsequent modifications may thus have contributed to the acquisition and diversification of gill covers and respiratory strategies during gnathostome evolution. well zebrafish dlx2a, This the jawless

引用

页码：24876 / 24884

页数：9

共 62 条

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