Enantioseparation of tropa alkaloids by means of anionic cyclodextrin-modified capillary electrophoresis

被引：0
|
作者：
Wedig, M [1 ]
Holzgrabe, U [1 ]
机构：
[1] Univ Bonn, Inst Pharmazeut, D-53115 Bonn, Germany
来源：
ELECTROPHORESIS | 1999年 / 20卷 / 07期
关键词：
tropa alkaloids; enantioseparation; negatively charged cyclodextrin;
D O I：
10.1002/(SICI)1522-2683(19990601)20:7<1555::AID-ELPS1555>3.0.CO;2-F
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Anionic cyclodextrins (CDs), i.e., sulfated CD, sulfobutylether beta-CD (SBE-beta-CD) and heptakis(2,3-di-O-acetyl-6-sulfato)-beta-CD (HDAS-beta-CD), were used to separate the isomers of atropine, homatropine, ipratropium, scopolamine and butylscopolamine. Variations in the pH value and the concentration of the various CDs revealed the sulfated beta-CD to be superior to the other derivatives. In a basic medium at low concentrations of sulfated CD the enantiomers of atropine, homatropine and ipratropium were well resolved in a short migration time, which is appropriate for the routine analysis of the enantiomeric excess. Scopolamine and butylscopolamine compounds could not be separated with either CD.
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页码：1555 / 1560
页数：6
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