CuO/WO3 nanoparticles decorated graphene oxide nanosheets with enhanced peroxidase-like activity for electrochemical cancer cell detection and targeted therapeutics

被引:62
作者
Alizadeh, Negar [1 ]
Salimi, Abdollah [1 ,2 ]
Hallaj, Rahman [1 ]
Fathi, Fardin [3 ]
Soleimani, Farzad [3 ]
机构
[1] Univ Kurdistan, Dept Chem, Sanandaj 6617715175, Iran
[2] Univ Kurdistan, Res Ctr Nanotechnol, Sanandaj 6617715175, Iran
[3] Kurdistan Univ Med Sci, Res Inst Hlth Dev, Cellular & Mol Res Ctr, Sanandaj 6617713446, Iran
来源
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2019年 / 99卷
关键词
Copper oxide-tungsten oxide nanoparticles; peroxidase like activity; Miniaturized electrochemical cell; Cancer cell detection; Targeted therapeutics; METAL-ORGANIC FRAMEWORKS; EFFICIENT PHOTOCATALYST; COLORIMETRIC DETECTION; COPPER NANOPARTICLES; CATALYTIC-ACTIVITY; GOLD NANOCLUSTERS; GLUCOSE; SENSOR; NANOCOMPOSITE; IMMUNOSENSOR;
D O I
10.1016/j.msec.2019.02.048
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
The novel method was developed for electrochemical cancer cell detection using CuO/WO3 nanoparticle decorated graphene oxide nanosheet (CuO/WO3-GO) with enhanced peroxidase like-activity, based on catalytic reaction of H2O2 with o-Phenylenediamine (OPD). The prepared nanocomposite conjugated with folic acid (FA), as a cancer cell-targeting ligand, and a miniaturized electrochemical cell for cancer cell detection was designed. In this strategy OPD could oxidize in the presence of H2O2 on the surface of working electrode, which produced an electrochemical signal. However, the redox response signal changed by interaction of cells with FA/CuO/WO3-GO. During interaction between cells and CuO/WO3-GO, some amount of H2O2-OPD system participated in chemical reaction and removed from the electrode, resulting in a decrease in the response signal. As a consequence, cancer cells detected in wide detection range of 50 to 10(5) cells/mL and a detection limit of 18 cells/mL. Furthermore, the nanocomposite shows therapeutic cancer treatment through superior peroxidase activity. This work unveils an effective method for simple, sensitive and selective monitoring of cancer cells and also has the potential for efficient cancer therapy, which will open an avenue of nanozymes toward biological applications.
引用
收藏
页码:1374 / 1383
页数:10
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