Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing

被引：1

作者：

Wu, Feima ^{[1
,2
,3
,4
]}

Gao, Ge ^{[1
,2
,3
]}

Pan, Tingcai ^{[1
,2
,3
,4
]}

Yang, Zhen ^{[1
,2
,3
,4
]}

Xu, Guosheng ^{[1
,2
,3
,4
]}

Abbas, Nasir ^{[1
,2
,3
,4
]}

Liu, Yanli ^{[1
,2
,3
,4
]}

Chen, Yan ^{[1
,2
,3
]}

Tan, Shenglin ^{[1
,2
,3
,4
]}

You, Kai ^{[1
,2
,3
]}

Ke, Xinrong ^{[1
,2
,3
,4
]}

Zhuang, Yuanqi ^{[1
,2
,3
]}

Lin, Xianhua ^{[1
,2
,3
]}

Yang, Fan ^{[1
,2
,3
]}

Li, Yin-xiong ^{[1
,2
,3
,4
,5
]}

机构：

[1] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Inst Publ Hlth, Guangzhou, Guangdong, Peoples R China

[2] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Biocomp, Guangzhou, Guangdong, Peoples R China

[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou, Guangdong, Peoples R China

[4] Univ Chinese Acad Sci, Beijing, Peoples R China

[5] Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou, Guangdong, Peoples R China

来源：

STEM CELL RESEARCH | 2018年 / 27卷

关键词：

D O I：

10.1016/j.scr.2017.12.002

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers. (c) 2017 The Authors. Published by Elsevier B.V.

引用

页码：5 / 9

页数：5

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