Brain-Derived Neurotrophic Factor Is Required for the Neuroprotective Effect of Mifepristone on Immature Purkinje Cells in Cerebellar Slice Culture

被引：5

作者：

Rakotomamonjy, Jennifer ^{[1
]}

Ghoumari, Abdel Mouman ^{[2
,3
,4
]}

机构：

[1] Northwestern Univ, Feinberg Sch Med, Dept Physiol, 303 East Chicago Ave, Chicago, IL 60611 USA

[2] INSERM, UMR 1195, 80 Rue Gen Leclerc, F-94276 Le Kremlin Bicetre, France

[3] Univ Paris Sud, 80 Rue Gen Leclerc, F-94276 Le Kremlin Bicetre, France

[4] Univ Paris Saclay, 80 Rue Gen Leclerc, F-94276 Le Kremlin Bicetre, France

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 02期

关键词：

Purkinje cells; mifepristone; BDNF; GABA(A) receptors; MAP-kinase; PROTEIN-KINASE; GABAERGIC TRANSMISSION; INDUCED APOPTOSIS; IN-VIVO; RAT; BDNF; ACTIVATION; TRKB; POTENTIATION; RELEASE;

D O I：

10.3390/ijms20020285

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Endogenous -aminobutyric acid (GABA)-dependent activity induces death of developing Purkinje neurons in mouse organotypic cerebellar cultures and the synthetic steroid mifepristone blocks this effect. Here, using brain-derived neurotrophic factor (BDNF) heterozygous mice, we show that BDNF plays no role in immature Purkinje cell death. However, interestingly, BDNF haploinsufficiency impairs neuronal survival induced by mifepristone and GABA(A)-receptors antagonist (bicuculline) treatments, indicating that the underlying neuroprotective mechanism requires the neurotrophin full expression.

引用

页数：9

共 41 条

[1] ACTIVITY-DEPENDENT PLASTICITY OF DEVELOPING CLIMBING FIBER-PURKINJE CELL SYNAPSES
Bosman, L. W. J.
Konnerth, A.
[J]. NEUROSCIENCE, 2009, 162 (03) : 612 - 623
[2] Requirement of TrkB for synapse elimination in developing cerebellar Purkinje cells
Bosman, Laurens W. J.
Hartmann, Jana
Barski, Jaroslaw J.
Lepier, Alexandra
Noll-Hussong, Michael
Reichardt, Louis F.
Konnerth, Arthur
[J]. BRAIN CELL BIOLOGY, 2006, 35 (01) : 87 - 101
[3] ERK1/2-mediated disruption of BDNF-TrkB signaling causes synaptic impairment contributing to fluoride-induced developmental neurotoxicity
Chen, Jingwen
Niu, Qiang
Xia, Tao
Zhou, Guoyu
Li, Pei
Zhao, Qian
Xu, Chunyan
Dong, Lixin
Zhang, Shun
Wang, Aiguo
[J]. TOXICOLOGY, 2018, 410 : 222 - 230
[4] PLCγ signaling underlies BDNF potentiation of Purkinje cell responses to GABA
Cheng, Q
Yeh, HH
[J]. JOURNAL OF NEUROSCIENCE RESEARCH, 2005, 79 (05) : 616 - 627
[5] Retrograde BDNF to TrkB signaling promotes synapse elimination in the developing cerebellum
Choo, Myeongjeong
Miyazaki, Taisuke
Yamazaki, Maya
Kawamura, Meiko
Nakazawa, Takanobu
Zhang, Jianling
Tanimura, Asami
Uesaka, Naofumi
Watanabe, Masahiko
Sakimura, Kenji
Kano, Masanobu
[J]. NATURE COMMUNICATIONS, 2017, 8
[6] Dusart I, 1997, J NEUROSCI, V17, P3710
[7] GABA-mediated Ca2+ signalling in developing rat cerebellar Purkinje neurones
Eilers, J
Plant, TD
Marandi, N
Konnerth, A
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 2001, 536 (02): : 429 - 437
[8] Estradiol increases expression of the brain-derived neurotrophic factor after acute administration of ethanol in the neonatal rat cerebellum
Firozan, Bita
Goudarzi, Iran
Salmani, Mahmoud Elahdadi
Lashkarbolouki, Taghi
Rezaei, Arezou
Abrari, Kataneh
[J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 2014, 732 : 1 - 11
[9] Neuroprotective effect of mifepristone involves neuron depolarization
Ghoumari, A. M.
Piochon, C.
Tomkiewicz, C.
Eychenne, B.
Levenes, C.
Dusart, I.
Schumacher, M.
Baulieu, E. E.
[J]. FASEB JOURNAL, 2006, 20 (09) : 1377 - 1386
[10] Mifepristone (RU486) protects Purkinje cells from cell death in organotypic slice cultures of postnatal rat and mouse cerebellum
Ghoumari, AM
Dusart, I
El-Etr, M
Tronche, F
Sotelo, C
Schumacher, M
Baulieu, EE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (13) : 7953 - 7958

← 1 2 3 4 5 →