Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenesis from Adipose-Derived Stem Cells

被引：2

作者：

Kim, Namyeong ^{[1
,2
]}

Choi, Joo Hee ^{[1
,2
]}

Choi, Min Joung ^{[1
,2
]}

Kim, Jin Su ^{[1
,2
]}

Kim, Wooyoup ^{[1
,2
]}

Song, Jeong Eun ^{[1
,2
]}

Khang, Gilson ^{[1
,2
]}

机构：

[1] Jeonbuk Natl Univ, Dept PolymerNano Sci & Technol, 567 Baekje Daero, Jeonju Si 54896, Jeonbuk, South Korea

[2] Jeonbuk Natl Univ, Polymer Mat Fus Res Ctr, Dept Bionanotechnol & Bioconvergence Engn, 567 Baekje Daero, Jeonju Si 54896, Jeonbuk, South Korea

来源：

MACROMOLECULAR RESEARCH | 2020年 / 28卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

adipose-derived stem cells; gellan gum; platelet-rich plasma; chondrogenesis; GELLAN GUM; IN-VITRO; CARTILAGE REGENERATION; PLASMA; DIFFERENTIATION; IMMOBILIZATION; PROLIFERATION; CHONDROCYTES; VIABILITY; MODEL;

D O I：

10.1007/s13233-020-8155-4

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

In this experiment, hydrogel composite was developed with gellan gum (GG) and platelet-rich plasma (PRP) to induce chondrogenesis of adipose-derived stem cells (ADSCs) in the hydrated environment. FTIR, SEM, and compression test were carried out to characterize the fabricated material. Biocompatibility was analyzed by MTT assay. The morphology of the encapsulated ADSCs was observed under SEM. Chondrogenesis was examined by PCR. The chemical and morphological analysis displayed the appearance of PRP in the matrix. The mechanical property of the hydrogel decreased as the PRP was loaded but viability and chondrogenesis were significantly increased with the proper amount of the PRP. Overall, the composite of the appropriate incorporation of PRP in the GG can enhance the microenvironment for chondrogenesis and expected to have great potential in tissue engineering.

引用

页码：1098 / 1103

页数：6

共 52 条

[1] A comparison of fibrin, agarose and gellan gum hydrogels as carriers of stem cells and growth factor delivery microspheres for cartilage regeneration [J].