Preparation of (1 → 4)-β-D-xylooligosaccharides from an acid hydrolysate of cotton-seed xylan:: suitability of cotton-seed xylan as a starting material for the preparation of (1 → 4)-β-D-xylooligosaccharides

被引:52
作者
Sun, HJ [1 ]
Yoshida, S [1 ]
Park, NH [1 ]
Kusakabe, I [1 ]
机构
[1] Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 3058572, Japan
关键词
xylan; cotton-seed; xylooligosaccharide;
D O I
10.1016/S0008-6215(02)00031-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cotton-seed residual cake, which is a byproduct of the process of oil extraction from the seed, was delignified with sodium hypochlorite (1% available chlorine). Xylan was then prepared from the delignified wet material by alkali extraction with 15% sodium hydroxide. The cotton-seed xylan contained 64.7% xylose and 9.4% uronic acid. The xylan was hydrolyzed with 0.125 M sulfuric acid at 90 degreesC for 15 min. The resultant hydrolysis products were separated by gel-permeation chromatography on BioGel P-4 and Toyopearl HW-40F columns connected in series, with water as an eluate. Xylose and xylooligosaccharides with a degree of polymerization ranging from DP 2 to 15 were separated under such conditions, and each xylooligosaccharide-containing peak fraction afforded a single band on fluorophore-assisted carbohydrate electrophoresis. These results suggest that cotton-seed xylan is suitable for the preparation of xylose and xylooligosaccharides. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:657 / 661
页数:5
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