DETERMINATION OF 8-HXYDROXY-2′DEOXYGUANOSINE IN URINE USING HPLC WITH ELECTROCHEMICAL DETECTION

被引：13

作者：

Domijan, Ana-Marija ^{[1
]}

Peraica, Maja ^{[1
]}

机构：

[1] Inst Med Res & Occupat Hlth, HR-10090 Zagreb, Croatia

来源：

ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY | 2008年 / 59卷 / 04期

关键词：

biomarker; free radicals; oxidative DNA damage; 8-oxodG;

D O I：

10.2478/10004-1254-59-2008-1879

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to DNA. Oxidative DNA damage is involved in the development of different diseases, aging, and cancer. Urine 8-hydroxy-2'deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the "whole body" oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in spot urine samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 50 nmol L-1 to 400 nmol L-1, the calibration curve was linear (rl=0.9995) and the limit of detection was 3 nmol L-1. Concentrations of 8-oxodG ranged between 3.0 nmol L-1 and 48.0 nmol L-1, which is in accordance with values reported for healthy individuals in other studies.

引用

页码：277 / 282

页数：6

共 10 条

[1] Methodology for urinary 8-hydroxy-2′-deoxyguanosine analysis by HPLC with electrochemical detection [J].

de Martinis, BS ;

Bianchi, MDLP .

PHARMACOLOGICAL RESEARCH, 2002, 46 (02) :129-131

[2] Separation procedures capable of revealing DNA adducts [J].

Esaka, Y ;

Inagaki, S ;

Goto, M .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 797 (1-2) :321-329

[3] Assay for the determination of urinary 8-hydroxy-2'deoxyguanosine by high-performance liquid chromatography with electrochemical detection [J].

Germadnik, D ;

Pilger, A ;

Rudiger, HW .

JOURNAL OF CHROMATOGRAPHY B, 1997, 689 (02) :399-403

[4]

Halliwell B, 2000, AM J CLIN NUTR, V72, P1082

[5] Estimation of oxidative DNA damage in man from urinary excretion of repair products [J].

Loft, S ;

Poulsen, HE .

ACTA BIOCHIMICA POLONICA, 1998, 45 (01) :133-144

[6] Oxidative DNA damage: Assessment of the role in carcinogenesis, atherosclerosis, and acquired immunodeficiency syndrome [J].

Olinski, R ;

Gackowski, D ;

Foksinski, M ;

Rozalski, R ;

Roszkowski, K ;

Jaruga, P .

FREE RADICAL BIOLOGY AND MEDICINE, 2002, 33 (02) :192-200

[7] Development of an HPLC method with electrochemical detection of femtomoles of 8-oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine in the presence of uric acid [J].

Rebelo, IA ;

Piedade, JAP ;

Oliveira-Brett, AM .

TALANTA, 2004, 63 (02) :323-331

[8] Determination of urinary 8-hydroxy-2′-deoxyguanosine in obese patients by HPLC with electrochemical detection [J].

Samcová, E ;

Marhol, P ;

Opekar, F ;

Langmaier, J .

ANALYTICA CHIMICA ACTA, 2004, 516 (1-2) :107-110

[9] Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics [J].

Wu, LL ;

Chiou, CC ;

Chang, PY ;

Wu, JT .

CLINICA CHIMICA ACTA, 2004, 339 (1-2) :1-9

[10] Study of urinary 8-hydroxydeoxyguanosine as a biomarker of oxidative DNA damage in diabetic nephropathy patients [J].

Xu, GW ;

Yao, QH ;

Weng, QF ;

Su, BL ;

Zhang, X ;

Xiong, JH .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2004, 36 (01) :101-104

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