Retention behaviour of an homologous series of oligodeoxythymidilic acids using reversed-phase ion-pair chromatography

被引:13
|
作者
McKeown, AP [1 ]
Shaw, PN [1 ]
Barrett, DA [1 ]
机构
[1] Univ Nottingham, Dept Pharmaceut Sci, Nottingham NG7 2RD, England
关键词
column liquid chromatography; ion-pair chromatography; oligonucleoticles; mobile phase pH;
D O I
10.1007/BF02491658
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The retention behaviour of an homologous series of phosphorylated oligodeoxythymidylic acid (pd(T)(5-18)) oligonucleotides was studied using reversed-phase ion-pair chromatography with isocratic elution conditions. The effects of temperature, pH, eluent ionic strength, percentage organic modifier, concentration and alkyl chain length of the ion-pairing reagent were investigated. The retention behaviour was generally explicable by current theoretical models of ion-pair chromatography However, the marked effect of mobile phase pH on the retention of the oligonucleotides was unexpected, and this was ascribed to the presence of ionisable residual silanols on the surface of the reversed-phase packing material.
引用
收藏
页码:271 / 277
页数:7
相关论文
共 50 条
  • [1] Retention behaviour of an homologous series of oligodeoxythymidilic acids using reversed-phase ion-pair chromatography
    A. P. McKeown
    P. N. Shaw
    D. A. Barrett
    Chromatographia, 2002, 55 : 271 - 277
  • [2] THE RETENTION EQUATION IN REVERSED-PHASE ION-PAIR CHROMATOGRAPHY
    LU, PC
    ZOU, HF
    ZHANG, YK
    MIKROCHIMICA ACTA, 1990, 3 (1-3) : 35 - 53
  • [3] EFFECT OF ION-PAIR REAGENT CONCENTRATION ON THE RETENTION IN REVERSED-PHASE ION-PAIR CHROMATOGRAPHY
    HONG, MF
    ZOU, HF
    ZHANG, YK
    LU, PZ
    ACTA CHIMICA SINICA, 1993, 51 (02) : 178 - 183
  • [4] RETENTION MECHANISM INVESTIGATION ON ION-PAIR REVERSED-PHASE CHROMATOGRAPHY
    AHUJA, S
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1985, 189 (APR-): : 124 - ANYL
  • [5] ELECTROSTATIC RETENTION MODEL OF REVERSED-PHASE ION-PAIR CHROMATOGRAPHY
    BARTHA, A
    STAHLBERG, J
    JOURNAL OF CHROMATOGRAPHY A, 1994, 668 (02) : 255 - 284
  • [6] ION-PAIR REVERSED-PHASE CHROMATOGRAPHY IN ANALYSIS OF PHENOL ACIDS
    PADALKINA, VS
    ARATSKOVA, AA
    YASHIN, YI
    KLYACHKO, YA
    ZHURNAL VSESOYUZNOGO KHIMICHESKOGO OBSHCHESTVA IMENI D I MENDELEEVA, 1981, 26 (04): : 474 - 476
  • [7] RETENTION MECHANISM FOR REVERSED-PHASE ION-PAIR LIQUID-CHROMATOGRAPHY
    BIDLINGMEYER, BA
    DEMING, SN
    PRICE, WP
    SACHOK, B
    PETRUSEK, M
    JOURNAL OF CHROMATOGRAPHY, 1979, 186 (DEC): : 419 - 434
  • [8] MODEL FOR RETENTION OF ANIONIC CHELATES IN ION-PAIR REVERSED-PHASE CHROMATOGRAPHY
    NIKITIN, YS
    BASOVA, EM
    BOLSHOVA, TA
    JOURNAL OF ANALYTICAL CHEMISTRY OF THE USSR, 1991, 46 (04): : 534 - 543
  • [9] REVERSED-PHASE ION-PAIR CHROMATOGRAPHY OF ANTHRACYCLINES
    VANLANCKER, MA
    NELIS, HJCF
    DELEENHEER, AP
    JOURNAL OF CHROMATOGRAPHY, 1983, 254 (JAN): : 45 - 52
  • [10] REVERSED-PHASE ION-PAIR CHROMATOGRAPHY OF OLIGODEOXYRIBONUCLEOTIDES
    SOKOLOWSKI, A
    BALGOBIN, N
    JOSEPHSON, S
    CHATTOPADHYAYA, JB
    SCHILL, G
    CHEMICA SCRIPTA, 1981, 18 (04): : 189 - 191