Environmental Regulation of Plant Gene Expression: An RT-qPCR Laboratory Project for an Upper-level Undergraduate Biochemistry or Molecular Biology Course

被引：8

作者：

Eickelberg, Garrett J. ^{[1
]}

Fisher, Alison J. ^{[1
]}

机构：

[1] Willamette Univ, Dept Chem, Salem, OR 97301 USA

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION | 2013年 / 41卷 / 05期

基金：

美国国家科学基金会;

关键词：

gene expression; laboratory exercises; molecular basis of cell and devel; biology; teaching and learning techniques methods and approaches; plant biochemistry; FLOWERING-LOCUS-C; REAL-TIME PCR; ARABIDOPSIS; FLC; VERNALIZATION; CHROMATIN; REPRESSOR; PROTEIN; DESIGN; SOC1;

D O I：

10.1002/bmb.20722

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a novel laboratory project employing real-time RT-qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires four 3-hr laboratory sessions and is aimed at upper-level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands-on experience with RT-qPCR, the current gold standard for gene expression analysis, including detailed data analysis using the common 2-CT method. Moreover, it provides a convenient starting point for many inquiry-driven projects addressing diverse questions concerning ecological biochemistry, naturally occurring genetic variation, developmental biology, and the regulation of gene expression in nature. (c) 2013 by The International Union of Biochemistry and Molecular Biology, 41(5):325-333, 2013

引用

页码：325 / 333

页数：9

共 32 条

[11] An Undergraduate Laboratory Experiment for Upper-Level Forensic Science, Biochemistry, or Molecular Biology Courses: Human DNA Amplification Using STR Single Locus Primers by Real-Time PCR with SYBR Green Detection [J].