Cloning, Expression, and Bioinformatics Analysis of Heavy Metal Resistance Gene afe_1862 from Acidithiobacillus ferrooxidans L1 in Escherichia coli

被引：5

作者：

Leng, Feifan ^{[1
]}

Li, Yuanli ^{[1
]}

Luo, Wen ^{[1
]}

Wei, Qingwei ^{[1
]}

Jing, Yanjun ^{[1
]}

Wang, Xiaoli ^{[2
]}

Yang, Mingjun ^{[1
]}

Wang, Yonggang ^{[1
]}

机构：

[1] Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China

[2] CAAS, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou 730050, Gansu, Peoples R China

来源：

BIOLOGICAL TRACE ELEMENT RESEARCH | 2019年 / 189卷 / 01期

关键词：

Acidithiobacillus ferrooxidans; Metal resistance; Expression; Bioinformatics; afe_1862; PROTEINS; COPPER; METALLOTHIONEIN; PREDICTION; PEPTIDES; CELL;

D O I：

10.1007/s12011-018-1462-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular studies of copper and cadmium resistances in acidophilic bacteria are significant in biomining. In this study, afe_1862, which encodes a heavy metal-binding protein in Acidithiobacillus ferrooxidans L1, was amplified using PCR, cloned into the pET32a plasmid, and sequenced. Following SDS-PAGE analysis, optimization of the expression conditions and heterologous overexpression of afe_1862 in Escherichia coli BL21 in the presence of Cu2+ and Cd2+ were studied as well. The results indicated that AFE_1862 has higher resistance to Cu2+ than Cd2+. Bioinformatics analysis illustrated that AFE_1862 has a conserved HMA domain containing heavy metal-binding sites, which may play a role in transporting or detoxifying heavy metals.

引用

页码：291 / 300

页数：10

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