PCR-mediated detection of acidophilic, bioleaching-associated bacteria

被引：36

作者：

DeWulfDurand, P ^{[1
]}

Bryant, LJ ^{[1
]}

Sly, LI ^{[1
]}

机构：

[1] UNIV QUEENSLAND, DEPT MICROBIOL, CTR BACTERIAL DIVERS & IDENTIFICAT, BRISBANE, QLD 4072, AUSTRALIA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 07期

关键词：

D O I：

10.1128/AEM.63.7.2944-2948.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The detection of acidophilic microorganisms from mining environments by culture methods is time consuming and unreliable. Several PCR approaches were developed to amplify small-subunit rRNA sequences from the DNA of six bacterial phylotypes associated with acidic mining environments, permitting the detection of the target DNA at concentrations as low as 10 fg.

引用

页码：2944 / 2948

页数：5

共 34 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] AN IMMUNOLOGICAL ASSAY FOR DETECTION AND ENUMERATION OF THERMOPHILIC BIOMINING MICROORGANISMS [J].