Properties and syntheses of copolymeric support for enzymes immobilization from vinylene carbonate and poly(vinylene alcohol) N-methylolarcrylamide ether

被引：7

作者：

Ding, LH ^{[1
]}

Li, Y ^{[1
]}

Jiang, Y ^{[1
]}

Cao, Z ^{[1
]}

Huang, JX ^{[1
]}

机构：

[1] Nankai Univ, Dept Chem, State Key Lab Funct Polymer Mat Adsorpt & Separat, Tianjin 300071, Peoples R China

来源：

JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY | 1999年 / A36卷 / 10期

关键词：

vinylene carbonate; poly(vinylene alcohol); N-methyloacrylamide; fixed enzyme; support; trypsin;

D O I：

10.1081/MA-100101607

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

First, Poly(vinylene alcohol) (PVA) N-methylolacrylamide ether (E-PVA) was prepared, then dissolved it with vinylene carbonate(VCA) and water-soluble initiator in water. The solution was copolymerized in paraffin oil to yield hydrophilic beads containing cyclic carbonate groups. By attachment of trypsin, properties of the supports were determined and the result shows that amount of enzymes coupled to supports and specific activity of immobilized trypsin were related to content of VCA structure unit, reaction time, concentration of enzyme solution, etc.

引用

页码：1443 / 1457

页数：15

共 23 条

[1]

ANGIURO LD, 1980, ANGEW MAKROMOL CHEM, V91, P161

[2] INVESTIGATIONS ON VINYLENE CARBONATE .4. RADIATION-INDUCED GRAFT-COPOLYMERIZATION OF VINYLENE CARBONATE AND N-VINYL-N-METHYLACETAMIDE ONTO POLYETHYLENE FILMS [J].