HSA-based phosphorescent probe for two-photon in vitro visualization

被引：9

作者：

Chelushkin, Pavel S. ^{[1
,2
]}

Nukolova, Natalia V. ^{[3
,4
]}

Melnikov, Alexei S. ^{[5
,6
]}

Serdobintsev, Pavel Yu. ^{[5
,6
]}

Melnikov, Pavel A. ^{[4
]}

Krupenya, Dmitry V. ^{[1
]}

Koshevoy, Igor O. ^{[7
]}

Burov, Sergey V. ^{[1
,2
]}

Tunik, Sergey P. ^{[1
]}

机构：

[1] St Petersburg State Univ, Inst Chem, St Petersburg 198504, Russia

[2] Russian Acad Sci, Inst Macromol Cpds, St Petersburg 199004, Russia

[3] Serbsky State Sci Ctr Social & Forens Psychiat, Moscow 119991, Russia

[4] Pirogov Russian Natl Res Med Univ, Moscow 117997, Russia

[5] St Petersburg State Univ, Dept Phys, St Petersburg 198504, Russia

[6] St Petersburg State Polytech Univ, Res Inst Nanobiotechnol, St Petersburg 195251, Russia

[7] Univ Eastern Finland, Dept Chem, Joensuu 80101, Finland

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2015年 / 149卷

基金：

俄罗斯基础研究基金会;

关键词：

Imaging agents; Luminescence; Non-linear optics; Non-covalent interactions; Human serum albumin; HUMAN SERUM-ALBUMIN; EXCITATION CROSS-SECTIONS; QUANTUM DOTS; LIVE CELLS; MICROSCOPY; COMPLEXES;

D O I：

10.1016/j.jinorgbio.2015.03.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-photon microscopy reveals several advantages over conventional one since it provides higher spatial resolution as well as deeper penetration into the sample under study. The development of suitable two-photon probes is one of the most challenging tasks in this area. Here we present phosphorescent non-covalent adduct of human serum albumin and Au-Ag alkynyl-diphosphine complex, [Au14Ag4(C2Ph)(12)(PPh(2)C(6)H(4)PPh2)(6)[PF6](4), which exhibits high cross section of two-photon-induced luminescence (delta(TPE)) within large near-infrared excitation wavelength region (700-800 nm) with maximum delta(TPE) about 38 GM at 740 nm. This feature makes it a promising probe for multiphoton bioimaging as demonstrated by successful visualization of glioma C6 cells and various tissues by two-photon confocal microscopy both in planar and z-stacking modes. Additionally, the broad excitation region enables optimization of the signal-to-background auto-fluorescence ratio via variation of excitation wavelength. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：108 / 111

页数：4

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