High-level production of thermotolerant β-xylosidase of Aspergillus sp BCC125 in Pichia pastoris: Characterization and its application in ethanol production

被引：21
作者：
Wongwisansri, Sriwan [1 ]
Promdonkoy, Peerada [1 ]
Matetaviparee, Panida [1 ]
Roongsawang, Niran [1 ]
Eurwilaichitr, Lily [1 ]
Tanapongpipat, Sutipa [1 ]
机构：
[1] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Bioresources Technol Unit, Klongluang 12120, Pathum Thani, Thailand
来源：
BIORESOURCE TECHNOLOGY | 2013年 / 132卷
关键词：
beta-xylosidase; Xylan hydrolysis; Pichia pastoris; Pichia stipitis; Ethanol fermentation; PAECILOMYCES-THERMOPHILA; PURIFICATION; EXPRESSION;
D O I：
10.1016/j.biortech.2012.11.117
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
A gene coding for thermotolerant beta-xylosidase from Aspergillus sp. BCC125 was characterized. The recombinant enzyme was expressed in methylotrophic yeast Pichia pastoris KM71 and especially high yield of secreted enzyme was obtained. beta-xylosidase possessed high enzyme efficiency (K-cat/K-m = 198.8 mM(-1) s(-1)) toward pNP-beta-D-xylopyranoside (pNP beta X) with optimal temperature and pH for activity of 60 degrees C and pH 4.0-5.0, respectively. The identified beta-xylosidase showed clear synergism with previously identified xylanase for hydrolysis of xylan in vitro as well as simultaneous saccharification and fermentation process (SSF) in vivo with Pichia stipitis. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：410 / 413
页数：4
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