ANALYSIS OF GENETIC VARIABILITY OF Psidium spp. (MYRTACEAE) ACCESS EVALUATED FOR RESISTANT TO Meloidogyne enterolobii

被引：3

作者：

Almeida, Eduardo Jose ^{[1
]}

Wickert, Ester

Santos, Jaime Maia ^{[2
]}

Gerald Martins, Antonio Baldo ^{[3
]}

机构：

[1] Univ Fed Sao Carlos, Dept Agroecol, Ctr Ciencias Agr, Araras, SP, Brazil

[2] UNESP, FCAV, Dept Prod Vegetal, Jaboticabal, SP, Brazil

[3] UNESP, FCAV, Dept Fitossanidade, Jaboticabal, SP, Brazil

来源：

REVISTA BRASILEIRA DE FRUTICULTURA | 2012年 / 34卷 / 02期

关键词：

disease resistance; tropical fruit; Psidium gua[!text type='java']java[!/text; root knot nematodes; genetic marker; ROOT-KNOT NEMATODE; MAYAGUENSIS;

D O I：

10.1590/S0100-29452012000200027

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

This study aimed to characterize molecular of 13 accessions of Psidium spp. (Myrtaceae) that was been identified for the reaction to rootknot guava nematode. The DNA extraction of the samples was carried according to the protocol of Shillito & Saul (1988). The molecular markers type fAFLP, were obtained from fAFLP Regular Plant Genomes Fingerprinting Kit' (Applied Biosystems from Brasil Ltda.) and were tested 24 selectives combinations of primers, of which 18 showed amplification that produced 272 polymorphic markers. To the analysis of the markers were employed the softwares GeneScan (ABI Prism versao 1.0) and Genotyper (ABI Prism version 1.03), and the data collected were transformed into a binary matrix that was analyzed in the software PAUP (Phylogenetic Analysis Using Parcimony - version 3.01). Were calculated genetic distance index intra and interespecific between the genotipes. It was found that the AFLP markers were efficient in the discrimination between accessions, as well as in showing genetic similarity among accessions identified as resistant to the nematode Meloidogyne enterolobii, which could be discussed in the future.

引用

页码：532 / 539

页数：8

共 20 条

[1]

ALMEIDA E.J., 2008, THESIS U ESTADUAL PA

[2] Resistance of guava and araca to Meloidogyne mayaguensis [J].

de Almeida, Eduardo Jose ;

dos Santos, Jaime Maia ;

Geraldo Martins, Antonio Baldo .

PESQUISA AGROPECUARIA BRASILEIRA, 2009, 44 (04) :421-423

[3]

[Anonymous], 1989, Molecular Cloning: A Laboratory

[4]

[Anonymous], [No title captured]

[5]

AUKAR A.P.A., 2003, THESIS U ESTADUAL PA

[6]

Carneiro Regina M. D. G., 2007, Fitopatologia Brasileira, V32, P281, DOI 10.1590/S0100-41582007000400001

[7]

FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x

[8]

Ferreira M.E, 1998, INTRO USO MARCADORES

[9]

Hartman H.T., 1997, Plant propagation: principles and practices, V6th

[10] MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment [J].

Kumar, S ;

Tamura, K ;

Nei, M .

BRIEFINGS IN BIOINFORMATICS, 2004, 5 (02) :150-163

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