DNA sperm damage correlates with nuclear ultrastructural sperm defects in teratozoospermic men

被引：27

作者：

Skowronek, F. ^{[1
,2
,3
]}

Casanova, G. ^{[2
,3
]}

Alciaturi, J. ^{[1
]}

Capurro, A. ^{[4
]}

Cantu, L. ^{[5
]}

Montes, J. M. ^{[5
]}

Sapiro, R. ^{[1
]}

机构：

[1] Sch Med, Dept Histol & Embryol, Lab Mol Biol Reprod, Montevideo 11800, Uruguay

[2] Sch Med, Dept Cellular & Mol Biol, Montevideo 11800, Uruguay

[3] Sch Med, Electron Microscopy Unit, Montevideo 11800, Uruguay

[4] Pereira Rossell Hosp Ctr, Human Reprod Unit, Montevideo, Uruguay

[5] Fertilab Canelones, Montevideo, Uruguay

来源：

ANDROLOGIA | 2012年 / 44卷 / 01期

基金：

美国国家卫生研究院;

关键词：

DNA damage; electron microscopy; male infertility; teratozoospermia; NOTULAE SEMINOLOGICAE; HUMAN SPERMATOZOA; PREDICTIVE-VALUE; MORPHOLOGY; FRAGMENTATION; INFERTILITY; FERTILITY; VACUOLES; TESTS; CELLS;

D O I：

10.1111/j.1439-0272.2010.01106.x

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Sperm morphology has consistently been the best indicator of male fertility. Transmission electron microscopy currently provides the most information on the subcellular details of sperm structure. Recently, assessment of sperm DNA damage has been employed to assess fertility potential. The purpose of this work was to link sperm DNA damage, evaluated by an intercalated fluorescent dye, with the structural characteristics of sperm. Conventional semen analysis was performed on samples from men undergoing fertility evaluation. Thirty men were evaluated and assigned to three subgroups based on strict criteria for sperm morphology: normal morphology (>14% normal forms), intermediate morphology (514% normal forms), and poor morphology (<5% normal forms). By quantifying acridine orange-positive cells and ultrastructural sperm defects, we found that the poor morphology pattern group showed a positive association between sperm carrying damaged DNA and the percentage of sperm nucleus with vacuoles (P = 0.01). No statistically significant correlations were established in other ultrastructural characteristics of sperm, including immature chromatin, lytic changes, or abnormal sperm tails. These results suggest that zones without chromatin in the sperm nucleus reflect underlying chromosomal or DNA defects in severe teratozoospermic men. This association should be considered in the evaluation of male fertility.

引用

页码：59 / 65

页数：7

共 33 条

[21] Sperm DNA Damage: Correlation to Severity of Semen Abnormalities [J].

Moskovtsev, Sergey I. ;

Willis, Jennifer ;

White, John ;

Mullen, Brendan M. .

UROLOGY, 2009, 74 (04) :789-793

[22]

Muratori M, 2000, J ANDROL, V21, P903

[23]

Ombelet Willem, 2003, Reprod Biomed Online, V7, P485

[24]

Organization WH, 2010, WHO LAB MAN EX PROC

[25] Quantitative evaluation of spermatozoa ultrastructure after aupuncture treatment for idiopathic male infertility [J].

Pei, J ;

Strehler, E ;

Noss, U ;

Abt, M ;

Piomboni, P ;

Baccetti, B ;

Sterzik, K .

FERTILITY AND STERILITY, 2005, 84 (01) :141-147

[26] Value of quantitative ultramorphological sperm analysis in infertile men [J].

Skowronek, Maria F. ;

Alciaturi, Jimena ;

Casanova, Gabriela ;

Capurro, Ana ;

Montes, Jose M. ;

Sapiro, Rossana .

REPRODUCTIVE BIOLOGY, 2010, 10 (02) :125-139

[27]

TEJADA RI, 1984, FERTIL STERIL, V42, P87

[28] The use of semen parameters to identify the subfertile male in the general population [J].

van der Merwe, FH ;

Kruger, TF ;

Oehninger, SC ;

Lombard, CJ .

GYNECOLOGIC AND OBSTETRIC INVESTIGATION, 2005, 59 (02) :86-91

[29]

WHO, 1999, WHO LAB MAN EX HUM S, V37, p[I, 1]

[30]

ZAMBONI L, 1987, FERTIL STERIL, V48, P711

← 1 2 3 4 →