A method for the analysis of low-mass molecules by MALDI-TOF mass spectrometry

被引：183

作者：

Guo, Z

Zhang, QC

Zou, HF ^{[1
]}

Guo, BC

Ni, JY

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China

[2] Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA

来源：

ANALYTICAL CHEMISTRY | 2002年 / 74卷 / 07期

关键词：

D O I：

10.1021/ac010979m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a novel method termed matrix suppressed laser desorption/ionization to improve the analysis of low-mass molecules by MALDI-TOF mass spectrometry. In this method, the surfactant of cetrimonium bromide (CTAB) is added to the conventional matrix of alpha-cyano-4-hydroxycinnamic acid solution to prepare the MALDI samples. During the MALDI process, the presence of CTAB could substantially or even completely suppress the matrix-related ion background. As a result, very clean mass spectra can be routinely obtained in the low-mass range. In addition, the presence of CTAB can significantly improve the mass resolution of low-mass molecules. It is seen that high-quality spectra were routinely obtained at a matrix/CTAB ratio of 1000:1. This method has been successfully used to analyze a variety of low-mass molecules.

引用

页码：1637 / 1641

页数：5

共 37 条

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