Transfection of the human malaria parasite Plasmodium falciparum

被引：29
作者：
Waterkeyn, JG [1 ]
Crabb, BS
Cowman, AF
机构：
[1] Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Div Infect & Immun, Parkville, Vic 3050, Australia
[2] Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia
来源：
INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1999年 / 29卷 / 06期
关键词：
Plasmodium; transfection; gene knock-outs; genome modification; integration; stable transformation; transient transformation;
D O I：
10.1016/S0020-7519(99)00047-8
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
In the past few years, methods have been developed which allow the introduction of exogenous DNA into the human malaria parasite Plasmodium falciparum. This important technical advance known as parasite transfection, provides powerful new tools to study the function of Plasmodium proteins and their roles in biology and disease. Already it has allowed the analysis of promoter function and has been successfully applied to establish the role of particular molecules and/or mutations in the biology of this parasite. This review summarises the current state of the technology and how it has been applied to dissect the function of the P. falciparum genome. (C) 1999 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
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收藏
页码：945 / 955
页数：11
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