Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability

被引：21

作者：

Xu, Xuemei ^{[1
]}

Fang, Shiqi ^{[1
]}

Zhuang, Yuan ^{[1
]}

Wu, Shanshan ^{[1
]}

Pan, Qingling ^{[1
]}

Li, Longjie ^{[1
]}

Wang, Xiaohui ^{[1
]}

Sun, Xueqing ^{[1
]}

Liu, Bifeng ^{[1
]}

Wu, Yuzhou ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Hubei Key Lab Bioinorgan Chem & Mat Med, Britton Chance Ctr Biomed Photon, Sch Chem & Chem Engn,Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China

[2] Max Planck Inst Polymer Res, Ackermannweg 10, D-55128 Mainz, Germany

来源：

MATERIALS | 2019年 / 12卷 / 06期

基金：

中国国家自然科学基金;

关键词：

cationic albumin; DNA origami; cellular uptake; stability; NANOSTRUCTURES; DELIVERY; BINDING;

D O I：

10.3390/ma12060949

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

DNA nanostructures, owing to their controllable and adaptable nature, have been considered as highly attractive nanoplatforms for biomedical applications in recent years. However, their use in the biological environment has been restricted by low cellular transfection efficiency in mammalian cells, weak stability under physiological conditions, and endonuclease degradation. Herein, we demonstrate an effective approach to facilitate fast transfection of DNA nanostructures and enhance their stability by encapsulating DNA origami with a biocompatible cationic protein (cHSA) via electrostatic interaction. The coated DNA origami is found to be stable under physiological conditions. Moreover, the cHSA coating could significantly improve the cellular transfection efficiency of DNA origami, which is essential for biological applications.

引用

页数：12

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