Real time atomic force fluorescence-microscopy on living cells

被引：1

作者：

Struckmeier, J ^{[1
]}

Klopp, E ^{[1
]}

Born, M ^{[1
]}

Hofmann, M ^{[1
]}

Rink, D ^{[1
]}

Jones, DB ^{[1
]}

Ecke, S ^{[1
]}

Butt, HJ ^{[1
]}

机构：

[1] Univ Marburg, Dept Semicond Phys, D-35032 Marburg, Germany

来源：

HYBRID AND NOVEL IMAGING AND NEW OPTICAL INSTRUMENTATION FOR BIOMEDICAL APPLICATIONS | 2001年 / 4434卷

关键词：

atomic force microscope (AFM); calcium dynamics; osteoblasts; mechanotransduction;

D O I：

10.1117/12.446671

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The reaction of bone cells after mechanical stimulation is a key issue in understanding the origins of osteoporosis. We investigate mechanical stimulation of osteoblasts and their reaction with a new combination of an atomic force apparatus with a fluorescence microscope. First measurements on the mechanically induced calcium response of osteoblasts are presented. The average threshold force for stimulation is 300nN and the dynamics of the calcium response is in the several tens of seconds range.

引用

页码：142 / 149

页数：8

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