The crystal structure of seabream antiquitin reveals the structural basis of its substrate specificity

被引：18

作者：

Tang, Wai-Kwan ^{[1
]}

Wong, Kam-Bo ^{[1
,2
]}

Lam, Yuk-Man ^{[1
]}

Cha, Sun-Shin ^{[3
]}

Cheng, Christopher H. K. ^{[1
,2
]}

Fong, Wing-Ping ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Hong Kong, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Ctr Prot Sci & Crystallog, Hong Kong, Hong Kong, Peoples R China

[3] Korea Ocean Res & Dev Inst, Marine Biotechnol & New Mat Res Div, Ansan, South Korea

来源：

FEBS LETTERS | 2008年 / 582卷 / 20期

关键词：

antiquitin; ALDH7; alpha-aminoadipic semialdehyde; x-ray crystallography; site-directed mutagenesis; pyridoxine-dependent epilepsy;

D O I：

10.1016/j.febslet.2008.07.059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of seabream antiquitin in complex with the cofactor NAD(+) was solved at 2.8 angstrom resolution. The mouth of the substrate-binding pocket is guarded by two conserved residues, Glu120 and Arg300. To test the role of these two residues, we have prepared the two mutants E120A and R300A. Our model and kinetics data suggest that antiquitin's specificity towards the substrate alpha-aminoadipic semialdehyde is contributed mainly by Glu120 which interacts with the alpha-amino group of the substrate. On the other hand, Arg300 does not have any specificity interaction with the alpha-carboxylate group of the substrate, but is important in maintaining the active site conformation. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3090 / 3096

页数：7

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